A comet assay of DNA damage and repair in K562 cells after photodynamic therapy using haematoporphyrin derivative, methylene blue and meso-tetrahydroxyphenylchlorin
| Autor: | Catharine M L West, James Moore, Fiona I. McNair, Brian Marples |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty DNA Repair DNA damage medicine.medical_treatment Photodynamic therapy Biology chemistry.chemical_compound Tumor Cells Cultured medicine Humans Hematoporphyrin Derivative Photosensitizer Electrophoresis Agar Gel Hematoporphyrin Gel electrophoresis Photosensitizing Agents Hyperthermia Induced Molecular biology Methylene Blue Comet assay Mesoporphyrins Photochemotherapy Oncology chemistry Leukemia Myeloid DNA Methylene blue DNA Damage Research Article |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/bjc.1997.295 |
| Popis: | Single-cell electrophoresis (comet assay) has been used to evaluate DNA damage and repair in the human myeloid leukaemia cell line K562 after low-dose (predominantly sub-lethal) treatments of hyperthermia and photodynamic therapy (PDT). Three different photosensitizers were examined: haematoporphyrin derivative (HpD), methylene blue (MB) and meso-tetrahydroxyphenylchlorin (mTHPC). None of the drugs in the absence of light, nor in light alone, resulted in detectable DNA damage. However, a significant amount of DNA damage was detected immediately after treatment with haematoporphyrin derivative or methylene blue PDT compared with drug-only or light-only treatments; no residual level of DNA damage was evident for either drug following a 4-h post-treatment incubation at 37 degrees C. No significant DNA damage was detected after meso-tetrahydroxyphenylchlorin PDT or hyperthermia either immediately or 4 h after treatment. We conclude that the alkaline comet assay can be applied as an effective screening assay for DNA damage induced by a range of laser therapies. |
| Databáze: | OpenAIRE |
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