An integrated pathway interaction network for the combination of four effective compounds from ShengMai preparations in the treatment of cardio-cerebral ischemic diseases
Autor: | Kai Shen, Yanni Lv, Yisha Tan, Kefeng Zhai, Boyang Yu, Hong-lin Chen, Junping Kou, Fang Li |
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Rok vydání: | 2015 |
Předmět: |
Ginsenosides
Ischemia Myocardial Ischemia Pharmacology medicine.disease_cause PC12 Cells Lignans Brain Ischemia Cell Line Brain ischemia Cyclooctanes medicine Animals Humans Pharmacology (medical) Cytokine Network Pathway Polycyclic Compounds Protein Interaction Maps business.industry Tumor Necrosis Factor-alpha Systems Biology Oxidative Stress Pathway NF-kappa B General Medicine Saponins medicine.disease Rats Drug Combinations Oxidative Stress Phosphorylation Tumor necrosis factor alpha Original Article Signal transduction business Oxidative stress Drugs Chinese Herbal Signal Transduction |
Zdroj: | Acta pharmacologica Sinica. 36(11) |
ISSN: | 1745-7254 |
Popis: | SMXZF (a combination of ginsenoside Rb1, ginsenoside Rg1, schizandrin and DT-13) derived from Chinese traditional medicine formula ShengMai preparations) is capable of alleviating cerebral ischemia-reperfusion injury in mice. In this study we used network pharmacology approach to explore the mechanisms of SMXZF in the treatment of cardio-cerebral ischemic diseases. Based upon the chemical predictors, such as chemical structure, pharmacological information and systems biology functional data analysis, a target-pathway interaction network was constructed to identify potential pathways and targets of SMXZF in the treatment of cardio-cerebral ischemia. Furthermore, the most related pathways were verified in TNF-α-treated human vascular endothelial EA.hy926 cells and H2O2-treated rat PC12 cells. Three signaling pathways including the NF-κB pathway, oxidative stress pathway and cytokine network pathway were demonstrated to be the main signaling pathways. The results from the gene ontology analysis were in accordance with these signaling pathways. The target proteins were found to be associated with other diseases such as vision, renal and metabolic diseases, although they exerted therapeutic actions on cardio-cerebral ischemic diseases. Furthermore, SMXZF not only dose-dependently inhibited the phosphorylation of NF-κB, p50, p65 and IKKα/β in TNF-α-treated EA.hy926 cells, but also regulated the Nrf2/HO-1 pathway in H2O2-treated PC12 cells. NF-κB signaling pathway, oxidative stress pathway and cytokine network pathway are mainly responsible for the therapeutic actions of SMXZF against cardio-cerebral ischemic diseases. |
Databáze: | OpenAIRE |
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