Chamber and microfluidic probe for microperfusion of organotypic brain slices
| Autor: | Arthur Queval, Nageswara R. Ghattamaneni, Raminder Gill, R. Anne McKinney, David Juncker, Cecile M. Perrault, Maryam Mirzaei |
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| Rok vydání: | 2010 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Microscope Materials science Confocal Transducers Microfluidics Biomedical Engineering Bioengineering Brain tissue Cellular level Sensitivity and Specificity Biochemistry law.invention Mice Organ Culture Techniques Slice preparation law Animals High resolution imaging Infusion Pumps Miniaturization Brain Reproducibility of Results Equipment Design General Chemistry Microfluidic Analytical Techniques Equipment Failure Analysis Computer-Aided Design Biomedical engineering |
| Zdroj: | Lab Chip. 10:326-334 |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/b916669f |
| Popis: | Microfluidic systems are increasingly being used for the culture and study of dissociated cells because they require only minute amounts of materials while enabling drug screening and chemotaxis studies down to the single cell level. However, the culture of organized tissue, such as brain slices, has been more difficult to adapt to microfluidic devices. Here, we present a microfluidic system, comprising (i) a perfusion chamber for the culture of organotypic slices that is compatible with high resolution imaging on inverted microscopes, and (ii) a novel transparent microfluidic probe (MFP) for the localized microperfusion of the brain tissue. The MFP is made in poly(dimethylsiloxane), features six micrometre-scale apertures and can be assembled within a few hours in a standard laboratory. Each aperture can indiscriminately be used either for the injection or aspiration of solutions, giving rise to many possible combinations. The MFP was successfully used for the perfusion of a small number of cells in a brain slice with concurrent confocal fluorescence imaging of the perfused dye and sub-cellular structures within the tissue. |
| Databáze: | OpenAIRE |
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