Antimicrobial activity of hypochlorite solutions and reciprocating instrumentation associated with photodynamic therapy on root canals infected with Enterococcus faecalis - An in vitro study
| Autor: | Caroline Tumelero Dias, Huriel Scartazzini Palhano, Vitor Hugo Sanches Menchik, José Antônio Poli de Figueiredo, Julia Zandoná, Matheus Albino Souza, Charise Dallazem Bertol, Luciana Grazziotin Rossato-Grando, Doglas Cecchin, Igor Paim Hoffmann |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
030103 biophysics Sodium Hypochlorite medicine.medical_treatment Biophysics Hypochlorite Photodynamic therapy Dermatology Enterococcus faecalis 03 medical and health sciences chemistry.chemical_compound Reciprocating motion 0302 clinical medicine medicine In vitro study Humans Pharmacology (medical) Calcium hypochlorite Photosensitizing Agents biology Dose-Response Relationship Drug 030206 dentistry Calcium Compounds biology.organism_classification Antimicrobial Combined Modality Therapy Bacterial Load Hypochlorous Acid Methylene Blue Oncology chemistry Photochemotherapy Sodium hypochlorite Dental Pulp Cavity Root Canal Preparation Nuclear chemistry |
| Zdroj: | Photodiagnosis and photodynamic therapy. 23 |
| ISSN: | 1873-1597 |
| Popis: | Introduction The aim of this study is to evaluate the antimicrobial activity of hypochlorite solutions and reciprocating instrumentation associated with photodynamic therapy (PDT). Methods One hundred and thirty two root canals were enlarged up to #35 K-file and inoculated with E.faecalis for 14 days. The 132 samples were randomly divided into eleven groups (n = 12) and subjected to the following protocols: G1-distilled water + Reciproc R40 (control), G2-1% sodium hypochlorite (NaOCl) + Reciproc R40, G3-2.5% NaOCl + Reciproc R40; G4-1% calcium hypochlorite (Ca[OCl]2) + Reciproc R40, G5-2.5% Ca(OCl)2 + Reciproc R40; G6-PDT; G7-distilled water + Reciproc R40 + PDT, G8-1% NaOCl + Reciproc R40 + PDT, G9-2.5% NaOCl + Reciproc R40 + PDT; G10-1% Ca(OCl)2 + Reciproc R40 + PDT, G11-2.5% Ca(OCl)2 + Reciproc R40 + PDT. The percentage bacterial reduction was checked by counting the colony-forming units (CFUs) in 10 samples of each group. The remaining 2 samples of each group were submitted to scanning electronic microscopy (SEM) to illustrate the effectiveness of the proposed treatments. Data were subjected to one-way ANOVA followed by Tukey’s post hoc test (α = 0.05). Results The greatest ability to promote bacterial reduction was observed in groups 8 (1% NaOCl + R40 + PDT), 9 (2.5% NaOCl + R40 + PDT), 10 (1% Ca[OCl]2 + R40 + PDT), and 11 (2.5% Ca[OCl]2 + R40 + PDT), with no significant difference between them (p Conclusions The association of PDT with hypochlorite solutions and reciprocating instrumentation provides effective elimination of E.faecalis. |
| Databáze: | OpenAIRE |
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