Replication of Epstein–Barr Virus within the Epithelial Cells of Oral Hairy Leukoplakia, an AIDS-Associated Lesion
Autor: | M.A. Conant, John S. Greenspan, Donald I. Abrams, Deborah Greenspan, Evelyne T. Lennette, U K Freese, Vibeke Petersen |
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Rok vydání: | 1985 |
Předmět: |
Male
Hairy leukoplakia Herpesvirus 4 Human Genes Viral viruses Biology Antibodies Viral Virus Replication medicine.disease_cause Epithelium Herpesviridae Virus Capsid medicine Humans Antigens Viral Papillomaviridae Leukoplakia Acquired Immunodeficiency Syndrome Oral hairy leukoplakia Virion Nucleic Acid Hybridization Homosexuality General Medicine medicine.disease Virology Epstein–Barr virus Tongue Neoplasms Herpes simplex virus Viral replication Immunoglobulin G DNA Viral Leukoplakia Oral |
Zdroj: | New England Journal of Medicine. 313:1564-1571 |
ISSN: | 1533-4406 0028-4793 |
Popis: | We conducted a study to identify the viruses in tissue specimens of oral "hairy" leukoplakia, a lesion that is found in immunosuppressed male homosexuals and that is associated with the subsequent development of the acquired immunodeficiency syndrome. When stained for papillomavirus core antigen, 49 of 67 biopsy specimens (73 per cent) yielded positive results in epithelial-cell nuclei. Electron microscopy showed papillomavirus-like particles in all of 25 specimens, and the herpes-type virus described in a previous report was seen in 23 of the 25 specimens. Three specimens had both types of particle in the same individual epithelial cells. Immunofluorescence for herpes simplex virus, varicella-zoster virus, and cytomegalovirus gave negative results in all cases, but 19 of 20 specimens showed intense nuclear staining in epithelial cells for the viral capsid antigen of Epstein-Barr virus (EBV). DNA hybridization using EBV probes in Southern blots demonstrated EBV DNA in all of 13 specimens and found 200 or more viral DNA molecules per cellular genome in 11 of the 13. The whole EBV genome was also demonstrated in the specimens and found to be in linear virion form. We conclude that EBV replicates within the epithelial cells in hairy leukoplakia. |
Databáze: | OpenAIRE |
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