Exploring frontiers of orbitrap performance for long transients
Autor: | Eugen Damoc, Alexander Makarov, Eduard Denisov |
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Jazyk: | angličtina |
Rok vydání: | 2023 |
Předmět: |
Resolution (mass spectrometry)
Mass spectrometry business.industry Chemistry 010401 analytical chemistry 010402 general chemistry Condensed Matter Physics Orbitrap 01 natural sciences Charge detection 0104 chemical sciences law.invention law Instrumentation (computer programming) Physical and Theoretical Chemistry Routing (electronic design automation) Process engineering business Instrumentation Spectroscopy |
Zdroj: | International Journal of Mass Spectrometry |
Popis: | Experiments on a modified Orbitrap Exploris instrument have shown that even in a compact instrument it is possible to achieve a resolving power in excess of 2,000,000 when appropriate tolerance and tuning requirements are met. Such levels are achieved for a 4-s detection time. A new Orbitrap design with improved pumping allowed, for the first time, isotopic resolution of intact monoclonal antibodies even when using standard nitrogen supply in the Ion Routing Multipole. Longer transients further improve signal-to-noise ratios, approaching levels needed for single-charge resolution in charge detection mass spectrometry. Further steps for improvement of instrumentation are discussed. |
Databáze: | OpenAIRE |
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