Strong cation-exchange chromatography of proteins on a sulfoalkylated monolithic cryogel
| Autor: | Massimo Morbidelli, Işık Perçin, Bastian Brand, Orhan Gezici, Rushd Khalaf |
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| Přispěvatelé: | Gezici, Orhan -- 0000-0001-6808-2282, [Percin, Isik -- Khalaf, Rushd -- Brand, Bastian -- Morbidelli, Massimo -- Gezici, Orhan] ETH, Inst Chem & Bioengn, CH-8093 Zurich, Switzerland -- [Gezici, Orhan] Nigde Univ, Fac Sci & Arts, Dept Chem, TR-51200 Nigde, Turkey -- [Percin, Isik] Hacettepe Univ, Div Mol Biol, Dept Biol, TR-06800 Ankara, Turkey, 0-Belirlenecek |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Monolithic HPLC column
Ion chromatography Sulfonic acid Biochemistry High-performance liquid chromatography Analytical Chemistry Separation Adsorption Cations Spectroscopy Fourier Transform Infrared Cation Exchange Resins Monolith Fourier transform infrared spectroscopy chemistry.chemical_classification geography geography.geographical_feature_category Chromatography Ion-exchange Ion exchange Chemistry Protein Organic Chemistry Cytochromes c Proteins General Medicine Chromatography Ion Exchange Chymotrypsinogen Gradient elution Microscopy Electron Scanning Methacrylates Muramidase Sulfonic Acids HPLC Yamamoto Model Cryogels |
| Popis: | WOS: 000350930200002 PubMed ID: 25683627 A new strong cation exchanger (SCX) monolithic column was synthesized by at-line surface modification of a cryogel prepared by copolymerization of 2-hydroxyethylmethacrylate (HEMA) and glycidyl-methacrylate (GMA). Sodium salt of 3-Mercaptopropane sulfonic acid (3-MPS) was used as the ligand to transform the surface of the monolith into a strong cation exchanger. The obtained material was characterized in terms of elemental analysis, infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Brunauer-Emmett-Teller (BET) N-2 adsorption, and used as a stationary phase for strong-cation exchange chromatography of some proteins, such as a-chymotrypsinogen, cytochrome c and lysozyme. Water permeability of the column was calculated according to Darcy's law (2.66 x 10(-13) m(2)). The performance of the monolithic cryogel column was evaluated on the basis of Height Equivalent to a Theoretical Plate (HETP). Retention behavior of the studied proteins was modeled on the basis of Yamamoto model to understand the role of the ion-exchange mechanism in retention behaviors. The considered proteins were successfully separated, and the obtained chromatogram was compared with that obtained with a non-functionalized cryogel column. (c) 2015 Elsevier B.V. All rights reserved. Scientific and Technological Research Council of Turkey (TUBITAK) [2219] O. Gezici would like to thank (i) Scientific and Technological Research Council of Turkey (TUBITAK) for the postdoctoral scholarship provided through 2219-program (2013-2014), and (ii) Nigde University (Turkey) for the facilities provided. |
| Databáze: | OpenAIRE |
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