Oxidative stress and genotoxicity of an organic and an inorganic nanomaterial to Eisenia andrei : SDS/DDAB nano-vesicles and titanium silicon oxide

Autor: Sónia Mendo, Filipe E. Antunes, Joana Lourenço, Verónica Nogueira, Sérgio Marques, Ruth Pereira, Maria G. Rasteiro, Bruno Correia, Ana Gavina
Rok vydání: 2017
Předmět:
Antioxidant
Health
Toxicology and Mutagenesis
medicine.medical_treatment
Glutathione reductase
Eisenia andrei
02 engineering and technology
010501 environmental sciences
medicine.disease_cause
01 natural sciences
Antioxidants
Lipid peroxidation
Soil
chemistry.chemical_compound
medicine
Animals
Soil Pollutants
Oligochaeta
0105 earth and related environmental sciences
Titanium
chemistry.chemical_classification
Glutathione Peroxidase
biology
Glutathione peroxidase
Public Health
Environmental and Occupational Health
Sodium Dodecyl Sulfate
General Medicine
Silicon Dioxide
021001 nanoscience & nanotechnology
biology.organism_classification
Biota
Pollution
Nanostructures
Quaternary Ammonium Compounds
Comet assay
Oxidative Stress
chemistry
Environmental chemistry
Comet Assay
Lipid Peroxidation
0210 nano-technology
Genotoxicity
Oxidative stress
DNA Damage
Zdroj: Ecotoxicology and Environmental Safety. 140:198-205
ISSN: 0147-6513
DOI: 10.1016/j.ecoenv.2017.02.035
Popis: In the past few years the number of studies on the toxic effects of nanomaterials (NMs) in the environment increased significantly. Nonetheless, the data is still scarce, since there is a large number of NMs and new ones are being developed each day. Soils are extremely important for life, and are easily exposed to the released NMs, thus enhanced efforts are needed to study the impacts on soil biota. The objective of the present work was to determine if different concentrations of two NMs, one inorganic (TiSiO4) and other organic (nano-vesicles of sodium sodecyl sulfate/ didodecyl dimethylammonium bromide - SDS/DDAB), are genotoxic to soil invertebrates. Additionally, it was intended to understand whether, in the event of occurring, genotoxicity was caused by the incapability of the cells to deal with the oxidative stress caused by these NMs. With that purpose, Eisenia andrei were exposed for 30 days to the artificial OECD soil contaminated with different concentrations of the NMs being tested. After the exposure, coelomocytes were extracted from earthworms and DNA damage was measured by the comet assay. The activity of antioxidant enzymes (e.g. glutathione peroxidase, glutathione reductase and glutathione-S-Transferase) and lipid peroxidation were also assessed. The results showed that both NMs were genotoxic, particularly TiSiO4 for which significant DNA damages were recorded for concentrations above 444mg of TiSiO4-NM/kg of soildw. Since no statistically significant differences were found in the tested antioxidant enzymes and in lipid peroxidation, the mechanism of genotoxicity of these NMs seemed to be unrelated with oxidative stress.
Databáze: OpenAIRE
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