Intra-population differences of apolipoproteins in the aqueous humor
| Autor: | David Bogorad, Parth A. Patel, Sai Karthik Kodeboyina, Wenbo Zhi, Garrett Jones, Shruti Sharma, Kathryn E. Bollinger, Tae Jin Lee, Ashok Sharma, Lane Ulrich, Amy Estes |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
Apolipoprotein E medicine.medical_specialty RC620-627 Low protein genetic structures Apolipoprotein B Endocrinology Diabetes and Metabolism Clinical Biochemistry Population Glaucoma Clinical nutrition Aqueous Humor APOA4 Sex Factors Endocrinology Tandem Mass Spectrometry Ophthalmology medicine Humans Tomography Optical Nutritional diseases. Deficiency diseases education Aged Aged 80 and over education.field_of_study biology business.industry Research Biochemistry (medical) Middle Aged medicine.disease eye diseases Race Factors Apolipoproteins Biological Variation Population biology.protein lipids (amino acids peptides and proteins) Female sense organs Sample collection business Glaucoma Open-Angle Tomography Optical Coherence Chromatography Liquid |
| Zdroj: | Lipids in Health and Disease Lipids in Health and Disease, Vol 20, Iss 1, Pp 1-10 (2021) |
| ISSN: | 1476-511X |
| Popis: | Background Evidence suggests that proteins related to lipid metabolism, such as apolipoproteins, play an important role in the maintenance of normal vision. While several members of the apolipoprotein family are abundant in human aqueous humor (AH), their study remains difficult due to the AH’s small volume, low protein concentration, and the invasive nature of sample collection. In this study, we report the use of Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) to discover associations between AH apolipoproteins and race, gender, and ocular structure in patients with and without primary open angle glaucoma (POAG). Methods AH samples were collected from 231 patients undergoing phacoemulsification or glaucoma incisional surgery at the Medical College of Georgia, Augusta University and subsequently analyzed via LC-MS/MS. The number of peptide spectrum matches (PSMs) for each protein was used as a semi-quantitative measure of relative protein levels. Parameters related to ocular structure were determined using Optical Coherence Tomography (OCT) and Heidelberg Retinal Tomography (HRT). These data sets were probed for relationships between apolipoprotein levels and POAG, demographics (gender and race), and ocular structure. Results A total of ten apolipoproteins were detected in the 231 collected AH samples, with six detected in 100% of the samples, one detected in almost 57% of the samples and three detected in less than 10% of the samples. The levels of APOA1, APOC3, and APOD were higher among POAG subjects. Stratification by gender and race revealed demographic-specific variations. The levels of five apolipoproteins (APOA1, APOA2, APOA4, APOC3, and APOD) were higher in female POAG patients, whereas no apolipoprotein levels were altered in male POAG patients. The levels of APOA1, APOA2, APOA4, and APOD were increased in glaucomatous African American patients, whereas APOE and APOH levels were decreased in glaucomatous Caucasian patients. We also found distinct associations between apolipoprotein levels and OCT and HRT parameters in patients with and without POAG. Conclusions The intra-population variation in apolipoprotein levels highlights the heterogeneity of glaucoma as a disease, suggesting the importance of personalized treatments. Gender and race-specific alterations may be associated with higher risks of POAG in females and members of the African American population. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink