MMP-2, MMP-9, and iNOS Expression in Human Dental Pulp Subjected to Orthodontic Traction
| Autor: | Maria Buscemi, Salvatore Provenzano, Angelo Leone, Giovanni Francesco Spatola, Carola Caradonna, A Mauro, Aldo Gerbino |
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| Přispěvatelé: | Leone, A, Mauro, A, Spatola, GF, Provenzano, S, Caradonna, C, Gerbino, A, Buscemi, M, Leone,A, Mauro,A, Spatola,GF, Provenzano,S, Caradonna,C, Gerbino,A, Buscemi,M |
| Rok vydání: | 2009 |
| Předmět: |
Male
Settore BIO/17 - Istologia Time Factors Nitric oxide synthetase Adolescent Tooth Movement Techniques Nitric Oxide Synthase Type II Dentistry Orthodontics Malocclusion Angle Class II Matrix metalloproteinase Nickel Female patient Orthodontic Wires Pressure Premolar Humans Medicine Bicuspid Child Titanium Odontoblasts MMP-2 Orthodontic wire business.industry medicine.disease Immunohistochemistry Biomechanical Phenomena Dental pulp iNOS medicine.anatomical_structure Matrix Metalloproteinase 9 Steel Matrix Metalloproteinase 2 Female Stress Mechanical Treatment time Malocclusion MMP-9 business Immunohistochemistry Dental pulp MMP-2 MMP-9 iNOS Dental Alloys Follow-Up Studies |
| Zdroj: | The Angle Orthodontist. 79:1119-1125 |
| ISSN: | 1945-7103 0003-3219 |
| DOI: | 10.2319/110308-557r.1 |
| Popis: | Objective: To test the hypothesis that some metalloproteinases (MMP-2, MMP-9) and inducible nitric oxide synthetase (iNOS) enzymes in dental pulp samples do not vary when subjected to orthodontic treatment. Materials and Methods: Human dental pulps were taken from male and female patients (N=10; age 10–14 years). A straight wire technique was used with nickel-titanium or steel archwires. The increase of pressure applied on teeth was gradual. Five patients were subjected to premolar extractions after 14 months of treatment and one after 24 months. Samples were Bouin-fixed, paraffin-embedded, and afterwards processed for immunohistochemistry using anti-MMP-2, anti-MMP-9, and anti-iNOS antibodies. Results: A reduction of MMP-2, MMP-9, and iNOS expression occurred in treated samples. This became more evident with increased treatment time. Conclusion: The hypothesis is rejected. The reduction of expression of those proteins revealed a time-dependent relationship. |
| Databáze: | OpenAIRE |
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