A mouse model for adenovirus gene delivery
Autor: | Lennart Philipson, Stephen Malin, Johannes Wilbertz, Kensaku Okamoto, Lorenz Poellinger, Sven Pettersson, Tiziano Tallone, Annika Samuelsson, Mitsue Miyahara |
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Rok vydání: | 2001 |
Předmět: |
Gene Expression Regulation
Viral Genetically modified mouse Cell type Myeloid Gene Transfer Horizontal Genes Viral Adenoviridae Infections Genetic enhancement Transgene Mice Transgenic Biology Gene delivery medicine.disease_cause Adenoviridae Mice In vivo medicine Animals Humans Multidisciplinary Gene Transfer Techniques Biological Sciences Molecular biology Cell biology Disease Models Animal medicine.anatomical_structure |
Zdroj: | Proceedings of the National Academy of Sciences. 98:7910-7915 |
ISSN: | 1091-6490 0027-8424 |
Popis: | The cellular attachment receptor for adenovirus (Ad), Coxsackie adenovirus receptor (CAR), required for delivery of Ad into primary cells, is not present on all cell types, thus restricting Ad-gene delivery systems. To circumvent this constrain, a transgenic mouse has been generated that expresses a truncated human CAR in all tissues analyzed. These mice allowed efficient in vitro infections at low multiplicities into lymphoid, myeloid, and endothelial cells. Furthermore, in vivo administration of Ad-vectors results in infection of macrophages, lymphocytes, and endothelial cells. In addition, tail vein injection resulted in targeting of virus into previously inaccessible areas, such as the lung and the capillaries of the brain. The CAR transgenic mice will be useful for rapid functional genomic analysis in vivo , for testing the efficacy of gene therapy procedures or as a source of easily transducible cells. |
Databáze: | OpenAIRE |
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