Changed mitochondrial function by pre- and/or postpartum diet alterations in sheep
| Autor: | Mette Olaf Nielsen, Ole Hartvig Mortensen, Wenche Jørgensen, Peter Schjerling, Christiane Gam, Niels Grunnet, Jesper Løvind Andersen, Morten Scheibye-Knudsen, Bjørn Quistorff |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Physiology Skeletal muscle mitochondria Endocrinology Diabetes and Metabolism Biopsy Muscle Fibers Skeletal Biology Mitochondrion DNA Mitochondrial Fetal Development Oxygen Consumption Pregnancy Physiology (medical) Internal medicine medicine Animals PPAR delta RNA Messenger Fetal programming Muscle Skeletal Sheep medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Uncoupling Agents Malnutrition High fat diet Maternal Nutritional Physiological Phenomena medicine.disease Mitochondria Muscle Endocrinology Animals Newborn Female Metabolic syndrome Function (biology) Transcription Factors |
| Zdroj: | American journal of physiology. Endocrinology and metabolism. 297(6) |
| ISSN: | 1522-1555 |
| Popis: | In a sheep model, we investigated diet effects on skeletal muscle mitochondria to look for fetal programming. During pregnancy, ewes were fed normally (N) or were 50% food restricted (L) during the last trimester, and lambs born to these ewes received a normal (N) or a high-fat diet (H) for the first 6 mo of life. We examined mitochondrial function in permeabilized muscle fibers from the lambs at 6 mo of age (adolescence) and after 24 mo of age (adulthood). The postpartum H diet for the lambs induced an ∼30% increase ( P < 0.05) of mitochondrial V̇o2maxand an ∼50% increase ( P < 0.05) of the respiratory coupling ratio (RCR) combined with lower levels of UCP3 and PGC-1α mRNA levels ( P < 0.05). These effects proved to be reversible by a normal diet from 6 to 24 mo of age. However, at 24 mo, a long-term effect of the maternal gestational diet restriction (fetal programming) became evident as a lower V̇o2max(∼40%, P < 0.05), a lower state 4 respiration (∼40%, P < 0.05), and lower RCR (∼15%, P < 0.05). Both PGC-1α and UCP3 mRNA levels were increased ( P < 0.05). Two analyzed muscles were affected differently, and muscle rich in type I fibers was more susceptible to fetal programming. We conclude that fetal programming, seen as a reduced V̇o2maxin adulthood, results from gestational undernutrition. Postnatal high-fat diet results in a pronounced RCR and V̇o2maxincrease in adolescence. However, these effects are reversible by diet correction and are not maintained in adulthood. |
| Databáze: | OpenAIRE |
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