Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain

Autor:	Padmaja Mehta-D’souza
Rok vydání:	2018
Předmět:	chemistry.chemical_classification chemistry.chemical_compound Schiff base Chromatography Chemistry Polyacrylamide Periodate Periodic acid–Schiff stain Glycoprotein Fluorescence Stain Polyacrylamide gel electrophoresis Molecular biology
Zdroj:	Methods in Molecular Biology ISBN: 9781493987443 Methods in Molecular Biology ISBN: 9781617798207
Popis:	Pro-Q Emerald 300 glycoprotein stain generates a bright-green fluorescent signal upon reacting with periodic acid-oxidized carbohydrate groups on proteins. With this dye, it is possible to detect proteins directly in the gel without the need to transfer them to a membrane. This dye is more sensitive than the standard periodic acid Schiff's base which uses acidic fuchsin dye.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c75e28b40a661c63fc014d711924d5cc https://doi.org/10.1007/978-1-4939-8745-0_14 Zobrazit plný text záznamu