A novel method of dynamic culture surface expansion improves mesenchymal stem cell proliferation and phenotype
| Autor: | Manuel Bueno, Lara Buscemi, Boris Hinz, Thomas M. Quinn, Pierre-Jean Wipff, Daniel Vonwil, Hicham Majd |
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| Předmět: |
Adult
Cellular differentiation Cell Culture Techniques Mesenchymal stem cell culture Clinical uses of mesenchymal stem cells Biology Bone-Marrow Infarcted Myocardium Marrow Stromal Cells Umbilical-Cord Blood Mesenchymal stem cell lineage Humans Cell Lineage Mesenchymal stem cell proliferation Progenitor cell Cell Proliferation Myofibroblast Tissue Stretch Mesenchymal stem cell Contact inhibition Differentiation Capacity Progenitor Cells Cell Differentiation Mesenchymal Stem Cells Cell Biology Cell biology Phenotype Cell culture In-Vitro Immunology Silicone Elastomers Molecular Medicine Muscle Developmental Biology |
| Popis: | Repeated passaging in conventional cell culture reduces pluripotency and proliferation capacity of human mesenchymal stem cells (MSC). We introduce an innovative cell culture method whereby the culture surface is dynamically enlarged during cell proliferation. This approach maintains constantly high cell density while preventing contact inhibition of growth. A highly elastic culture surface was enlarged in steps of 5% over the course of a 20-day culture period to 800% of the initial surface area. Nine weeks of dynamic expansion culture produced 10-fold more MSC compared with conventional culture, with one-third the number of trypsin passages. After 9 weeks, MSC continued to proliferate under dynamic expansion but ceased to grow in conventional culture. Dynamic expansion culture fully retained the multipotent character of MSC, which could be induced to differentiate into adipogenic, chondrogenic, osteogenic, and myogenic lineages. Development of an undesired fibrogenic myofibroblast phenotype was suppressed. Hence, our novel method can rapidly provide the high number of autologous, multipotent, and nonfibrogenic MSC needed for successful regenerative medicine. |
| Databáze: | OpenAIRE |
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