Microsatellite instability affecting the T17 repeats in intron 8 of HSP110, as well as five mononucleotide repeats in patients with colorectal carcinoma
| Autor: | Zoran Krivokapic, Ivan Dimitrijevic, Srdjan Markovic, Daniela Bojic, Petar Svorcan, Jadranka Antic, Branimir Zogovic, Velimir Markovic |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Proto-Oncogene Proteins B-raf Colorectal cancer Clinical Biochemistry Biology MLH1 medicine.disease_cause Proto-Oncogene Proteins p21(ras) 03 medical and health sciences 0302 clinical medicine Proto-Oncogene Proteins Drug Discovery medicine Humans Allele HSP110 Heat-Shock Proteins neoplasms 030304 developmental biology Adaptor Proteins Signal Transducing Ovum 0303 health sciences Polymorphism Genetic Biochemistry (medical) Intron Microsatellite instability Nuclear Proteins DNA Methylation Middle Aged medicine.disease Spermatozoa digestive system diseases Introns 030220 oncology & carcinogenesis Mutation Cancer research ras Proteins Microsatellite Female Microsatellite Instability KRAS Carcinogenesis Colorectal Neoplasms MutL Protein Homolog 1 Microsatellite Repeats |
| Zdroj: | Biomarkers in medicine. 7(4) |
| ISSN: | 1752-0371 |
| Popis: | Aim: To investigate mononucleotide markers: BAT-25, BAT-26, NR-21, NR-22 and NR-24 in patients with colorectal cancer (CRC), and the status of HSP110T17, KRAS, BRAF and the MLH1 promoter mutations in microsatellite unstable CRC. Methods: Genetic assessments were performed on samples obtained following resection of CRC in 200 patients. Results: Allelic variations of HSP110T17 were found in all 18 patients with microsatellite instabilities (MSIs) in at least three markers (high-frequency MSI). By contrast, mutations of HSP110T17 were absent in all 20 patients with no MSI frequency. Eight out of 182 patients with low (instability in one marker) or no frequency MSI had allelic shifts due to polymorphisms of BAT-25 (1.5%), NR-21 (1.75%) and NR-24 (1.5%). BRAF mutations were associated with >5 bp shortening of HSP110T17. Conclusion: Patients with high-frequency MSI CRC had allelic variations of HSP110T17. BRAF mutations occur along with greater shortening in HSP110T17 during oncogenesis via the MSI pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|