Yiqi Huoxue prescription can prevent and treat post-MI myocardial remodeling through promoting the expression of AMPK signal pathway
| Autor: | Xi Chen, Wenchen Liu, Yuqin Zhang, Shuwen Guo, Zhaoduo Su, Jiangong Wu, Lu Zhang, Mingjing Zhao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
YQHX prescription 030204 cardiovascular system & hematology Mitochondrion Pharmacology 03 medical and health sciences Ginseng 0302 clinical medicine Perindopril medicine Myocardial infarction lcsh:Miscellaneous systems and treatments Ejection fraction biology business.industry AMPK biology.organism_classification medicine.disease lcsh:RZ409.7-999 Mitochondria Coronary arteries ATP Astragalus 030104 developmental biology medicine.anatomical_structure Complementary and alternative medicine business medicine.drug |
| Zdroj: | Journal of Traditional Chinese Medical Sciences, Vol 4, Iss 3, Pp 235-244 (2017) |
| ISSN: | 2095-7548 |
| Popis: | Objective: To investigate how Yiqi Huoxue (YQHX) prescription regulates mitochondrial biosynthesis and ATP synthesis via AMP-activated protein kinase (AMPK) and to reveal its molecular mechanism in preventing and treating post-MI myocardial remodeling. Methods: The MI animal model of myocardial infarction were established by ligating Sprague Dawley (SD) rats' left anterior descending coronary arteries; the animals were randomly divided into MI group, YQHX prescription group and perindopril group, and a sham operation group was set at the same time. Related drug intervention was administered on the 2nd day after surgery, the YQHX group was given Astragalus, angelica, ginseng, Ligusticum wallichii and pseudoginseng, provided by the Dongzhimen Hospital of Beijing University of Chinese Medicine, once per day, at a dose of 21 g/kg body weight/day (the clinical equivalent dose based on a previous study), and the changes in relevant indicators were observed at 1 week and 4 weeks. Echocardiography (ECG) was used to observe the changes in rat cardiac structure and functions; the morphology-based technique was used to observe the changes in myocardial cells and mitochondria; the expression of AMPK signal pathway-related proteins and mRNA was detected using western blotting and real-time fluorescence quantification respectively, while fluorescence enzyme-labeled method was used for detecting ATP synthesis. Results: Cardiac structure and functions: Compared with the MI group at 1 week, the YQHX prescription group and the perindopril group exhibited increased LVEF and LVFS (Pall .05). At 4 weeks, both LVEF and LVFS were elevated in the YQHX prescription group (P = .008, .009) and the perindopril group (P = .279, .333), where differences in the later group indicated no statistical significance; the YQHX prescription group and the perindopril group were also featured by reduced LVEDs and LVEDd (Pall |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|