Identification of centromere regions in chromosomes of a unicellular red alga, Cyanidioschyzon merolae
| Autor: | Motomichi Matsuzaki, Kan Tanaka, Yu Kanesaki, Sousuke Imamura |
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| Rok vydání: | 2015 |
| Předmět: |
Genetics
ChIP-chip assay Whole genome tiling array biology Cyanidioschyzon merolae In silico Centromere Biophysics Chromosome Cell Biology Genome project Sequence Analysis DNA biology.organism_classification Plant cell Biochemistry Chromosomes Plant Evolution Molecular Structural Biology Rhodophyta Identification (biology) CENP-A Molecular Biology |
| Zdroj: | FEBS letters. 589(11) |
| ISSN: | 1873-3468 |
| Popis: | To investigate the evolution of centromere architecture in plant cells, it is important to identify centromere regions of primitive algae, such as Cyanidioschyzon merolae. In a previous genome project, in silico analysis predicted an AT-rich region in each chromosome as putative centromere regions. Here, we identified a centromere position in each chromosome by ChIP-on-chip analysis using an anti-CENP-A antibody. The identified centromeres were of the regional type, about 2–3kb in length and contained no consensus or repeat elements. Centromeres in primitive eukaryotic plant cells may have originated from these regional type centromeres. |
| Databáze: | OpenAIRE |
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