Preliminary study on chromosomal aberrations in eggs of mice fertilized in vitro after X-irradiation
| Autor: | Akiko Ohkawa, Yoichi Matsuda, Izuo Tobari, Takeshi Yamada |
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| Rok vydání: | 1983 |
| Předmět: |
Male
Mice Inbred Strains Fertilization in Vitro Biology Insemination Andrology Mice Prophase Human fertilization Animals Irradiation Metaphase reproductive and urinary physiology Ovum Genetics Chromosome Aberrations Mice Inbred ICR Zygote urogenital system Cell Cycle General Medicine Sperm Spermatozoa In vitro Blastocyst Fertilization embryonic structures Female |
| Zdroj: | Mutation research. 121(2) |
| ISSN: | 0027-5107 |
| Popis: | The X-ray induction of chromosomal aberrations in the eggs of mice fertilized in vitro was studied by using a fertilization technique developed by Yamada et al. (1982a,b). Mature sperm, eggs just before insemination and zygotes at the early pronuclear stage were irradiated with 40 R of X-rays. In a control and two groups of irradiated sperm or unfertilized eggs, most of the 1-cell eggs progressed to the metaphase stage (92.2–94.4%) at 15–17 h after insemination. In the group irradiated at the early pronuclear stage, 38.2% of the 1-cell eggs were still at the pronuclear stage; furthermore, a delay of chromatin condensation in one of the pro-nuclei was often observed. The frequency of chromosomal aberrations in the irradiated zygotes (20.6%) was much higher than that in the irradiated sperm (2.9%) or in unfertilized eggs (11.0%). |
| Databáze: | OpenAIRE |
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