The rutin/β-cyclodextrin interactions in fully aqueous solution: spectroscopic studies and biological assays
| Autor: | Stefania Catania, Valentina Villari, Paola Ficarra, Chiara Costa, Rosanna Stancanelli, D. Raneri, Rita Ficarra, Paola Donato, Silvana Tommasini, Calabrò Ml |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Circular dichroism
Rutin Clinical Biochemistry Pharmaceutical Science Analytical Chemistry chemistry.chemical_compound Ultraviolet visible spectroscopy Drug Discovery Leukocytes Animals Humans Organic chemistry Drug Interactions Solubility Spectroscopy chemistry.chemical_classification Aqueous solution Cyclodextrin Circular Dichroism beta-Cyclodextrins 1HNMR Binding constant Batophenanthroline test Lipid peroxidation Molecular encapsulation Rats cyclodextrin 1H NMR Uv-vis CD binding constant solubility measurements Solutions Pharmaceutical Solutions chemistry Spectrophotometry Ultraviolet Comet Assay Lipid Peroxidation Phenanthrolines Nuclear chemistry |
| Zdroj: | Journal of pharmaceutical and biomedical analysis 36 (2005): 1019–1027. doi:10.1016/j.jpba.2004.09.018 info:cnr-pdr/source/autori:M.L. Calabrò; S. Tommasini; P. Donato; R. Stancanelli; D. Raneri; S. Catania; C. Costa; V. Villari; P. Ficarra; R. Ficarra/titolo:The rutin%2Fbeta-cyclodextrin interactions in fully aqueous solution: spectroscopic studies and biological assays/doi:10.1016%2Fj.jpba.2004.09.018/rivista:Journal of pharmaceutical and biomedical analysis (Print)/anno:2005/pagina_da:1019/pagina_a:1027/intervallo_pagine:1019–1027/volume:36 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2004.09.018 |
| Popis: | In the present work the feasibility of β-cyclodextrin complexation was explored, as a tool for improving the aqueous solubility and antioxidant efficacy of rutin. By means of 1 H NMR, UV–vis and circular dichroism spectroscopy the single aromatic ring of rutin was found to be inserted into the β-cyclodextrin cavity to form a 1:1 inclusion complex. The effect of β-cyclodextrin on the spectral features of rutin was quantitatively investigated, in fully aqueous medium, by holding the concentration of the guest constant and varying the host concentration. The associated binding constants were estimated to be 142 ± 20 and 153 ± 20 M −1 , respectively, on the basis of the observed UV–vis absorption and circular dichroism intensities. The antioxidant activity of rutin was also investigated, as affected by molecular encapsulation within β-cyclodextrin (batophenanthroline test; comet assay; lipid peroxidation); the inclusion complex revealed improved antioxidant efficacy that may be in part explained by an increased solubility in the biological moiety. |
| Databáze: | OpenAIRE |
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