Proteins of the Thermus thermophilus ribosome. Purification of proteins from the large ribosomal subunit
| Autor: | I.A. Kashparov, L.F. Markova, Muranova Ta, M. B. Garber, O.S. Shikaeva, S.E. Sedelnikova, N.K. Avlijakulov |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Ribosomal Proteins
Molecular Sequence Data Biochemistry Ribosome Ribosomal protein Large ribosomal subunit Electrophoresis Gel Two-Dimensional Amino Acid Sequence Peptide sequence chemistry.chemical_classification biology Molecular mass Sequence Homology Amino Acid Thermus thermophilus General Medicine Reversed-phase chromatography biology.organism_classification Chromatography Ion Exchange Molecular biology Amino acid Molecular Weight chemistry Spectrophotometry Ultraviolet Ribosomes |
| Zdroj: | Biochimie. 76(5) |
| ISSN: | 0300-9084 |
| Popis: | Special procedures have been developed to isolate and purify 26 of the 30 individual proteins of the large ribosomal subunit from Thermus thermophilus. Sixteen of them have been purified under non-denaturing conditions to be used for crystallization and further structural studies. These proteins have been characterized by their amino acid content, molecular mass, UV-spectrum and extinction coefficient. An additional 10 proteins have been purified by reverse phase chromatography. Thirteen proteins have been identified by homological E coli proteins. |
| Databáze: | OpenAIRE |
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