Aquaporin 9 expression and its localization in normal skeletal myofiber
| Autor: | Hiroko Kojima, Seiji Shibuya, Yoko Matsuzaki, Shoji Iijima, Takahiro Jimi, Hiroaki Oniki, Masahiko Inoue, Hajime Hara, Yoshihiro Wakayama, Hisatsugu Masaki |
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| Rok vydání: | 2009 |
| Předmět: |
Histology
Physiology Immunoblotting Muscle Fibers Skeletal Immunocytochemistry Aquaporin Biology Aquaporins Antibodies Mice medicine Animals Humans Myocyte Gel electrophoresis Messenger RNA Reverse Transcriptase Polymerase Chain Reaction Skeletal muscle Cell Biology General Medicine Immunohistochemistry Molecular biology Mice Inbred C57BL Protein Transport medicine.anatomical_structure Real-time polymerase chain reaction Gene Expression Regulation |
| Zdroj: | Journal of Molecular Histology. 40:165-170 |
| ISSN: | 1567-2387 1567-2379 |
| Popis: | The examination was performed whether aquaporin (AQP) 9 is expressed in normal skeletal muscle at mRNA and protein levels. Gel electrophoresis of the reverse transcription-polymerase chain reaction (RT-PCR) product of total RNA samples of human normal muscles by oligonucleotide primers for human AQP9 showed a band of 221 basepairs, which corresponded to the basepair length between two primers of AQP9. The nucleotide sequence of RT-PCR product coincided with that of human AQP9. Immunoblot analysis revealed that the rabbit and sheep antibodies against the synthetic peptide of the C-terminal cytoplasmic domain of human AQP9 molecule reacted with a protein of approximately 30 kDa molecular weight in extracts of human normal skeletal muscles. Immunohistochemistry with our anti-AQP9 antibodies showed an immunoreaction at the myofiber surface of both type 1 and type 2 fibers with almost equal staining intensity in human skeletal muscles. The implication of AQP9 expression in skeletal myofibers was discussed. |
| Databáze: | OpenAIRE |
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