Effects of recombinant human gastric lipase and pancreatin duringin vitropediatric gastro-intestinal digestion
| Autor: | Sofie Falkenløve Madsen, Ragna Berthelsen, Morten F. Ebbesen, Xiaolu Geng, Christine Heerup, Anette Müllertz |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
law.invention 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Digestion (alchemy) law Phosphatidylcholine Humans Gastric lipase Lipase Triglyceride lipase 030109 nutrition & dietetics Chromatography biology digestive oral and skin physiology Infant Newborn Infant 04 agricultural and veterinary sciences General Medicine 040401 food science digestive system diseases In vitro chemistry Pancreatin biology.protein Recombinant DNA Digestion Infant Food Lipid digestion Food Science |
| Zdroj: | Heerup, C, Ebbesen, M F, Geng, X, Madsen, S F, Berthelsen, R & Müllertz, A 2021, ' Effects of recombinant human gastric lipase and pancreatin during in vitro pediatric gastro-intestinal digestion ', Food & Function, vol. 12, no. 7, pp. 2938-2949 . https://doi.org/10.1039/D0FO02976A Heerup, C, Ebbesen, M F, Geng, X, Madsen, S F, Berthelsen, R & Müllertz, A 2021, ' Effects of recombinant human gastric lipase and pancreatin during in vitro pediatric gastro-intestinal digestion ', Food & Function, vol. 12, no. 7, pp. 2938-2949 . https://doi.org/10.1039/d0fo02976a |
| ISSN: | 2042-650X 2042-6496 |
| DOI: | 10.1039/d0fo02976a |
| Popis: | The aim of the study was to implement a gastric digestion step using recombinant human gastric lipase (rHGL) in an in vitro pediatric gastro-intestinal digestion model to achieve a physiologically relevant gastric contribution to total gastro-intestinal lipid digestion. A commercial infant formula (NAN Comfort stage 1 (NAN1)) with 3.4% lipid and an in-lab prepared oil-in-water emulsion, emulsified with soy phosphatidylcholine (SPCemul), with 3.5% lipid (oil-blend containing Akonino NS, MEG-3 and ARASCO oils) were subjected to in vitro gastro-intestinal digestion. To achieve a physiologically relevant level of gastric digestion, 50 min of in vitro gastric digestion, using either 0, 3.75 or 7.5 TBU mL-1 rHGL, was followed by 90 min of in vitro intestinal digestion, using either 0 or 26.5 TBU mL-1 pancreatic triglyceride lipase (PTL) from porcine pancreatin. The digestion of the substrates was assessed using titration-based quantification supported by HPLC-ELSD analysis. In vitro gastric digestion of NAN1 and SPCemul with either 3.75 or 7.5 TBU mL-1 rHGL contributed with 10-27% of the total gastro-intestinal digestion, corresponding to the reported contribution in human infants. At the end of the gastro-intestinal digestion (t = 140 min), the combined lipolytic effect of rHGL and PTL was additive during digestion of SPCemul, but not for the digestion of NAN1, as all lipase activity combinations resulted in a similar degree of NAN1 digestion. The effect of gastric digestion with rHGL on total digestion therefore appeared to be substrate dependent. To conclude, a gastric digestion step using rHGL resulting in physiologically relevant gastric contribution to the observed gastro-intestinal digestion was successfully implemented into an in vitro pediatric gastro-intestinal digestion model. |
| Databáze: | OpenAIRE |
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