The F420H2-dehydrogenase from Methanolobus tindarius: cloning of the ffd operon and expression of the genes in Escherichia coli
| Autor: | Claudia Ruppert, Michael Blaut, Christina Herzberg, Gerhard Gottschalk, Volker Müller, David J. Westenberg, Annett Braune |
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| Rok vydání: | 1999 |
| Předmět: |
Operon
Sequence analysis Archaeal Proteins Molecular Sequence Data medicine.disease_cause Microbiology Genes Archaeal 03 medical and health sciences Open Reading Frames Genetics medicine Escherichia coli Amino Acid Sequence Cloning Molecular Molecular Biology Peptide sequence 030304 developmental biology 0303 health sciences biology Base Sequence 030302 biochemistry & molecular biology Nucleic acid sequence Methanolobus Methanosarcinaceae biology.organism_classification Molecular biology Recombinant Proteins Methanolobus tindarius Coenzyme F420 hydrogenase Biochemistry biology.protein Oxidoreductases |
| Zdroj: | FEMS microbiology letters. 170(2) |
| ISSN: | 0378-1097 |
| Popis: | The membrane-bound F420H2-dehydrogenase from the methylotrophic methanogen Methanolobus tindarius oxidizes reduced coenzyme F420 and feeds the electrons into an energy-conserving electron transport chain. Based on the N-terminal amino acid sequence of the 40-kDa subunit of F420H2-dehydrogenase the corresponding gene ffdB was detected in chromosomal DNA of M. tindarius. Sequence analysis, primer extension, and RT-PCR experiments indicated that ffdB is part of an operon harboring three additional open reading frames (ffdA, ffdC, ffdD). The corresponding mRNA transcript and transcription start sites were determined. All four genes could be heterologously expressed in Escherichia coli. |
| Databáze: | OpenAIRE |
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