Evaluation of Tagetes patula (Asteraceae) as an ecological alternative in the search for natural control of the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)
| Autor: | R. R. Fantatto, Maria Izabel Camargo-Mathias, Alexander Alves da Silva, Maysa Furlan, Bruno Rodrigues Sampieri, Isabela Jacob Moro, Amanda Figueiredo, Flávio Augusto Sanches Politi, Ana Carolina de Souza Chagas |
|---|---|
| Přispěvatelé: | Flávio Augusto Sanches Politi, UNESP, Maysa Furlan, UNESP., Rafaela Regina Fantatto, UNESP, Alexander Alves da Silva, UNESP, Isabela Jacob Moro, UNESP, Bruno Rodrigues Sampieri, UNICAMP, Maria Izabel Camargo-Mathias, UNESP, Amanda Figueiredo, UNESP, ANA CAROLINA DE SOUZA CHAGAS, CPPSE, Universidade Estadual Paulista (Unesp), Universidade Estadual de Campinas (UNICAMP), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Keratinocytes Nymph Veterinary medicine Livestock 01 natural sciences 030308 mycology & parasitology Cell Line Tagetes 03 medical and health sciences Ticks Oils Volatile Rhipicephalus Animals Humans Acari Ectoparasitic control Acaricides Ovum Microscopy 0303 health sciences Natural products Germinal vesicle Ecology biology Tick Control Acaricide Plant Extracts fungi Ovary General Medicine biology.organism_classification Carrapato 010602 entomology Animal ecology Insect Science Larva Rhipicephalus microplus Female Animal parasites and pests Tagetes patula Ixodidae Spotted fever |
| Zdroj: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2019-10-06T17:09:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-04-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The cattle tick Rhipicephalus microplus is a great sanitary problem and causes huge losses to livestock, being a vector of important diseases. The aim of this work was to verify the action of plant-derived material obtained from Tagetes patula on the life cycle of R. microplus, as well as to analyze the action of these extracts on ovary cells of engorged females. In the Adult Immersion Test, the crude ethanolic extract (TpEtOH) showed an efficacy of 99.2% (LC50 = 18.60 mg mL−1). From the repellency test, it was found that both the TpEtOH and essential oil were 100% efficient on the larval stage. In addition, in the Larval Package Test, a mortality of 98.37% was obtained with TpEtOH (LC50 = 3.798 mg mL−1). From the microscopic analysis of ovary cells, morphological changes in the chorion and cytoplasm of oocytes were observed, with vacuolization around the germinal vesicle and disorganization of the pedicular cells, suggesting an interference in the normal embryogenic formation of the larvae. These results demonstrate that T. patula extracts interfere at all stages of development of the ixodid, from eggs to adults forms, and have a pronounced repellent effect. In addition, the results of the cytotoxicity assays performed on keratinocytes, as well as previous information on oral and dermal acute toxicity (LD50 > 4000 mg kg−1), attest that T. patula can be a safe alternative for ectoparasitic control, representing an alternative for development of a formulation used to help control populations of ticks in the field. Instituto de Química de Araraquara Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Faculdade de Ciências Farmacêuticas de Araraquara Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Museu de Zoologia Universidade Estadual de Campinas (UNICAMP) Instituto de Biociências de Rio Claro Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Empresa Brasileira de Pesquisa Agropecuária Embrapa Pecuária Sudeste Instituto de Química de Araraquara Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Faculdade de Ciências Farmacêuticas de Araraquara Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Instituto de Biociências de Rio Claro Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) CNPq: 150302/2017-7 FAPESP: 2013/03493-8 FAPESP: 2013/07600-3/CIBFar |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|