In vitro interactions of 51Cr in human red blood cells and hemolysates
| Autor: | Charles P. Emerson, Donald R. Harkness, Eugene M. Skrabut, Duane A. Bechthold, C. Robert Valeri, Gary V. Kuehl |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Chromium
Chromate conversion coating Chemistry Sodium Glucosephosphates chemistry.chemical_element Hematology General Medicine Erythrocyte Aging Hemolysis In vitro Chromium Radioisotopes Citric Acid Sickle Cell Trait Glucose Biochemistry Chlorides Chromium Compounds Chromatography Gel Distribution (pharmacology) Humans Hemoglobin Chromatography Thin Layer Citrates Intracellular |
| Zdroj: | Vox sanguinis. 40(4) |
| ISSN: | 0042-9007 |
| Popis: | The intracellular distribution of radioactivity was studied in normal and sickle erythrocytes labeled with sodium 51 Cr chromate. Both types of cells had a higher fraction of 51 Cr bound to hemoglobin when labeled in the presence of ACD at a pH of 7.09 than when labeled in the presence of CPD at a pH of 5.96. Citrate at a pH of 5.96 or less entered the red blood cells and decreased the 51Cr binding hemoglobin. Binding of 51Cr to hemoglobin within the red cells was also reduced when the ATP and DPG levels in the red blood cells were elevated. Studies with hemoglobin solution showed that 51Cr binding to hemoglobin was influenced by 2,3-DPG, ATP and citrate. |
| Databáze: | OpenAIRE |
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