Morphological comparison and functional reconstitution of rat hepatic parenchymal cells on various matrices
| Autor: | Ryoichi Awata, Toshihiro Sugiyama, Katsuyuki Imai, Kunihiko Terada, Hiroshi Sawai, Haruki Senoo |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Pathology medicine.medical_specialty Biology Extracellular matrix 03 medical and health sciences 0302 clinical medicine In vivo Parenchyma medicine Animals Rats Wistar Cells Cultured Cell Aggregation Matrigel Hepatology Gastroenterology Extracellular Matrix Rats Cell biology Drug Combinations Microscopy Electron medicine.anatomical_structure Liver 030220 oncology & carcinogenesis Hepatocyte Microscopy Electron Scanning Proteoglycans 030211 gastroenterology & hepatology Collagen Laminin Liver function Softgel Gels Type I collagen medicine.drug |
| Zdroj: | Journal of gastroenterology and hepatology. 13(S1) |
| ISSN: | 1440-1746 |
| Popis: | Four types of materials, type I collagen coat (Coat), acid-soluble type I collagen gel (Hardgel), pepsin-treated acid-soluble type I collagen gel (Softgel), and an extract of extracellular matrix of the murine Engelbreth-Holm-Swarm sarcoma (Matrigel), were used as matrices to culture rat hepatic parenchymal cells, and their morphological changes and adhesion were compared to the matrices by electron microscopic observations. Hepatic parenchymal cells cultured on Coat and Hardgel were extended and flattened, whereas cells cultured on Softgel and Matrigel assembled and formed aggregates. Such aggregates consisted of several hepatic parenchymal cells, with a recognizable bile duct-like alveolus on the inside. Morphologically, the aggregates were more spherical on Matrigel and oval shaped on Softgel. Microvilli of the cell surface were parallel to the matrix on Matrigel, but invaded into the gel on Softgel. Subsequently, investigation into how these morphological features affected the liver-specific functions, including secretion of albumin and induction of P450 by 3-methylcholanthrene, demonstrated that a high level of liver function was maintained in a long-term culture in hepatic parenchymal cells on Softgel. These results suggest that hepatic parenchymal cell interactions were stronger with Softgel than with Matrigel, and that Softgel appears to closely mimic the in vivo environment. |
| Databáze: | OpenAIRE |
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