Sex pheromone activity in a single component of tergal gland extract ofLutzomyia longipalpis (Diptera: Psychodidae) from Jacobina, Northeastern Brazil

Autor:	M. J. Dougherty, James G. C. Hamilton, R. D. Ward
Rok vydání:	1994
Předmět:	Hplc analysis Chromatography Single component General Medicine Biology biology.organism_classification Biochemistry High-performance liquid chromatography Small peak Sex pheromone Botany Bioassay Psychodidae Gas chromatography Ecology Evolution Behavior and Systematics
Zdroj:	Journal of Chemical Ecology. 20:141-151
ISSN:	1573-1561 0098-0331
DOI:	10.1007/bf02065997
Popis:	The sex pheromone component of maleLutzomyia longipalpis tergal gland extract was isolated and its activity confirmed by bioassay. Whole tergal gland extract was analyzed by HPLC and fractions were collected as they eluted from the detector. Each fraction was tested in an attraction bioassay with virgin unfed femaleLutzomyia longipalpis. HPLC analysis showed that whole extract contained several peaks; one large peak, one small peak and several minor peaks. Purity of the HPLC fractions was determined by GC analysis. The bioassays revealed that the large peak was responsible for most of the observed female behavior. The addition of the small peak to the large peak improved the response although by itself the small peak failed to elicit any significant behavior. Minor peaks failed to elicit any response. Chemical analysis revealed the large peak to be a relatively nonpolar hydrocarbon.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c73e211ab6577d7176a0b8d6da87ee71 https://doi.org/10.1007/bf02065997 Zobrazit plný text záznamu Full text from SpringerLink