Cellular phosphorylation of neurofilament heavy-chain by cyclin-dependent kinase-5 masks the epitope for monoclonal antibody N52

Autor:	Christopher C.J. Miller, Narinder P. S. Bajaj, Sonia Guidato
Rok vydání:	1996
Předmět:	Neurofilament COS cells Kinase medicine.drug_class General Neuroscience Cyclin-dependent kinase 5 Biology Monoclonal antibody Molecular biology Epitope Cyclin-dependent kinase medicine biology.protein Phosphorylation biological phenomena cell phenomena and immunity
Zdroj:	Neuroscience Letters. 217:157-160
ISSN:	0304-3940
DOI:	10.1016/s0304-3940(96)13098-6
Popis:	N52 is a widely used monoclonal antibody reported to recognise both phosphorylated and non-phosphorylated forms of neurofilament (NF)-H. N52 is therefore classified as a phosphorylation-independent-type antibody. N52 is strongly reactive with NF-H in COS cells transfected with NF-H alone but co-transfection of NF-H with the neurofilament kinase cdk-5 and one of its activators p35, induced phosphorylation of NF-H that abolished this reactivity. Treatment of the cdk-5 phosphorylated NF-H with alkaline phosphatase so as to remove phosphate restored N52 reactivity. A fragment of NF-H containing the consensus cdk-5 sites was reactive with N52 but following co-transfection with cdk-5/p35 a slower migrating fragment species generated by cdk-5 was not labelled by N52. These results demonstrate that N52 is not a truly phosphorylation-independent-type NF-H antibody and suggest that the N52 epitope contains sites targeted for phosphorylation by cdk-5.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c73ce9977a9d301a5fdbbd3907570fbe https://doi.org/10.1016/s0304-3940(96)13098-6 Zobrazit plný text záznamu Full Text from ScienceDirect