Redistribution of Ionotropic Glutamate Receptors Detected by Laser Microdissection of the Rat Dentate Gyrus 48 h following LTP Induction In Vivo
| Autor: | Sara E. Mason-Parker, Wickliffe C. Abraham, Jeremy T. T. Kennard, Diane Guévremont, Joanna M. Williams |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Time Factors Physiology Long-Term Potentiation lcsh:Medicine Mechanical Treatment of Specimens Rats Sprague-Dawley Learning and Memory Cell Signaling Tubulin Molecular Cell Biology LTP induction Membrane Receptor Signaling lcsh:Science Long-term depression Mammals Multidisciplinary musculoskeletal neural and ocular physiology Neurotransmitter Receptor Signaling Long-term potentiation Animal Models Cell biology Electrophysiology Protein Transport Electroporation medicine.anatomical_structure Specimen Disruption Biochemistry Vertebrates Research Article Signal Transduction Laser Capture Microdissection AMPA receptor Biology Research and Analysis Methods Receptors N-Methyl-D-Aspartate Rodents Model Organisms medicine Animals Receptors AMPA Dentate gyrus lcsh:R Organisms Biology and Life Sciences Cell Biology Perforant path Rats Protein Subunits Gene Expression Regulation nervous system Specimen Preparation and Treatment Cellular Neuroscience Dentate Gyrus Synapses Synaptic plasticity lcsh:Q Molecular Neuroscience Postsynaptic density Neuroscience |
| Zdroj: | PLoS ONE, Vol 9, Iss 3, p e92972 (2014) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0092972 |
| Popis: | The persistence and input specificity of long-term potentiation (LTP) make it attractive as a mechanism of information storage. In its initial phase, both in vivo and in vitro studies have shown that LTP is associated with increased membrane localization of AMPA receptor subunits, but the molecular basis of LTP maintenance over the long-term is still unclear. We have previously shown that expression of AMPA and NMDA receptor subunits is elevated in whole homogenates prepared from dentate gyrus 48 h after LTP induction in vivo. In the present study, we utilized laser microdissection (LMD) techniques to determine whether AMPA and NMDA receptor upregulation occurs specifically in the stimulated regions of the dentate gyrus dendritic arbor. Receptor proteins GluN1, GluA1 and GluA2, as well as postsynaptic density protein of 95 kDa and tubulin were detected by Western blot analysis in microdissected samples. Gradients of expression were observed for GluN1 and GluA2, decreasing from the inner to the outer zones of the molecular layer, and were independent of LTP. When induced at medial perforant path synapses, LTP was associated with an apparent specific redistribution of GluA1 and GluN1 to the middle molecular layer that contains these synapses. These data indicate that glutamate receptor proteins are delivered specifically to dendritic regions possessing LTP-expressing synapses, and that these changes are preserved for at least 48 h. |
| Databáze: | OpenAIRE |
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