Nitric oxide modulates the immunological response of bovine PBMCs in an in vitro BRDc infection model
| Autor: | Selvarani Vimalanathan, Gilly Regev-Shoshani, Chris Miller, Michael Peter Sheridan, James Martins |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Bovine Respiratory Disease Complex Inflammation Nitric Oxide Peripheral blood mononuclear cell Proinflammatory cytokine Microbiology 03 medical and health sciences Immune system medicine Animals Mannheimia haemolytica Cells Cultured Herpesvirus 1 Bovine Innate immune system General Veterinary biology biology.organism_classification Bovine herpesvirus 1 030104 developmental biology Gene Expression Regulation Immunology TLR4 Leukocytes Mononuclear Cytokines Tumor necrosis factor alpha Cattle medicine.symptom |
| Zdroj: | Research in veterinary science. 109 |
| ISSN: | 1532-2661 |
| Popis: | Bovine respiratory disease complex (BRDc) is a multi-factorial disease, involving both viral and bacterial pathogens, that negatively impacts the cattle feedlot industry. A nitric oxide releasing solution (NORS) has been developed and shown to have potential in the prevention of BRDc. This study investigated the underlying immunological mechanisms through which the nitroslyating agent NORS provides protection against the development of BRDc in susceptible cattle. An in vitro BRDc experimental model was designed using bovine peripheral blood mononuclear cells (PBMCs) which were infected with bovine herpesvirus 1 (BHV-1) and subsequently cultured with lipopolysaccharides (LPS) extracted from Mannheimia haemolytica bacteria. The cells were treated with NORS following viral infection to reflect the timing of administering the NORS treatment in feedlots during initial processing. An expression and protein analysis of key genes involved in the innate immune response was carried out. The BRDc model produced significant increases in gene expression (p |
| Databáze: | OpenAIRE |
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