Intravital imaging-based analysis tools for vessel identification and assessment of concurrent dynamic vascular events
| Autor: | Bàrbara Laviña, Naoki Honkura, Miguel Sáinz-Jaspeado, Mark Richards, Lena Claesson-Welsh, Christer Betsholtz |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Vascular Endothelial Growth Factor A Injections Intradermal Cell- och molekylärbiologi Science Green Fluorescent Proteins General Physics and Astronomy Gene Expression Mice Transgenic General Biochemistry Genetics and Molecular Biology Article Green fluorescent protein 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Genes Reporter Microscopy medicine Animals Intradermal injection Claudin-5 Promoter Regions Genetic skin and connective tissue diseases lcsh:Science Multidisciplinary Chemistry General Chemistry Blood flow Molecular Imaging Mice Inbred C57BL Red blood cell Vascular endothelial growth factor A 030104 developmental biology medicine.anatomical_structure Dextran Microscopy Fluorescence Multiphoton Flow velocity Biophysics Blood Vessels Female lcsh:Q sense organs 030217 neurology & neurosurgery Cell and Molecular Biology Blood Flow Velocity |
| Zdroj: | Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-018-04929-8 |
| Popis: | The vasculature undergoes changes in diameter, permeability and blood flow in response to specific stimuli. The dynamics and interdependence of these responses in different vessels are largely unknown. Here we report a non-invasive technique to study dynamic events in different vessel categories by multi-photon microscopy and an image analysis tool, RVDM (relative velocity, direction, and morphology) allowing the identification of vessel categories by their red blood cell (RBC) parameters. Moreover, Claudin5 promoter-driven green fluorescent protein (GFP) expression is used to distinguish capillary subtypes. Intradermal injection of vascular endothelial growth factor A (VEGFA) is shown to induce leakage of circulating dextran, with vessel-type-dependent kinetics, from capillaries and venules devoid of GFP expression. VEGFA-induced leakage in capillaries coincides with vessel dilation and reduced flow velocity. Thus, intravital imaging of non-invasive stimulation combined with RVDM analysis allows for recording and quantification of very rapid events in the vasculature. Different stimuli can induce dynamic changes in blood flow velocity, vessel diameter and permeability. Here the authors develop a multi-photon microscopy-based image analysis tool allowing the identification of vessels and the assessment of rapid changes in large vascular networks. |
| Databáze: | OpenAIRE |
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