Initial interaction of apoA-I with ABCA1 impacts in vivo metabolic fate of nascent HDL*s⃞
| Autor: | Xian-Cheng Jiang, Soonkyu Chung, Perry L. Colvin, Elena Boudyguina, John S. Parks, Ji-Young Lee, Anny Mulya, Thomas L. Smith, Abraham K. Gebre |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Apolipoprotein B lecithin:cholesterol acyltransferase Protein Conformation Mice Transgenic QD415-436 High-Density Lipoproteins Pre-beta ABCA1 transporter Biochemistry Cell Line Mice Endocrinology In vivo Phospholipid transfer protein Internal medicine Cell Line Tumor medicine Animals Humans Cells Cultured Kidney biology Apolipoprotein A-I Catabolism Cell Biology In vitro phospholipid transfer protein Rats Mice Inbred C57BL medicine.anatomical_structure Cell culture ABCA1 biology.protein in vivo catabolism ATP-Binding Cassette Transporters lipids (amino acids peptides and proteins) high density lipoproteins ATP Binding Cassette Transporter 1 Research Article |
| Zdroj: | Journal of Lipid Research, Vol 49, Iss 11, Pp 2390-2401 (2008) |
| ISSN: | 0022-2275 |
| Popis: | We investigated the in vivo metabolic fate of pre-beta HDL particles in human apolipoprotein A-I transgenic (hA-I (Tg)) mice. Pre-beta HDL tracers were assembled by incubation of [(125)I]tyramine cellobiose-labeled apolipoprotein A-I (apoA-I) with HEK293 cells expressing ABCA1. Radiolabeled pre-beta HDLs of increasing size (pre-beta1, -2, -3, and -4 HDLs) were isolated by fast-protein liquid chromatography and injected into hA-I (Tg)-recipient mice, after which plasma decay, in vivo remodeling, and tissue uptake were monitored. Pre-beta2, -3, and -4 had similar plasma die-away rates, whereas pre-beta1 HDL was removed 7-fold more rapidly. Radiolabel recovered in liver and kidney 24 h after tracer injection suggested increased (P < 0.001) liver and decreased kidney catabolism as pre-beta HDL size increased. In plasma, pre-beta1 and -2 were rapidly ( |
| Databáze: | OpenAIRE |
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