Distribution of Chlamydia trachomatis serotypes in clinical urogenital samples from north-eastern Croatia
| Autor: | Ivana Roksandić Križan, Nataša Ružman, Jelena Burazin, Magdalena Perić, Arlen Antolović-Požgain, Zinka Bošnjak, Snježana Džijan, Dinko Pavlinić, Gordan Lauc, Dubravka Vuković |
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| Rok vydání: | 2011 |
| Předmět: |
Serotype
Adult Male Adolescent Genotype Croatia Chlamydia trachomatis prevalence serotype distribution Omp1 gene sequence analysis Prevalence Porins Biology Urine medicine.disease_cause Real-Time Polymerase Chain Reaction Applied Microbiology and Biotechnology Microbiology Polymorphism Single Nucleotide law.invention Young Adult law Seroepidemiologic Studies medicine Humans Genitalia Serotyping Direct fluorescent antibody Polymerase chain reaction Aged Aged 80 and over Lymphogranuloma venereum General Medicine Middle Aged medicine.disease Virology Real-time polymerase chain reaction Fluorescent Antibody Technique Direct Lymphogranuloma Venereum Female |
| Zdroj: | Current microbiology. 64(6) |
| ISSN: | 1432-0991 |
| Popis: | The purpose of this study was to determine prevalence of Chlamydia trachomatis (Ct) urogenital infection and its serotype distribution from clinical samples in north-eastern Croatia. During a 3-year period, 2, 379 urogenital samples were analyzed by real-time polymerase chain reaction (A group), while 4, 846 genital swabs were analyzed by direct fluorescent antibody test (B group). 132 Ct positive specimens were genotyped by omp1 gene sequencing. The prevalence rate of Ct was 3.2 % in A and 1 % in B group. The most prevalent chlamydial genotype was E (44 %), followed by F (33 %), K (11.5 %), G (8 %), J/UW (5.3 %), D-IC (4.4 %), D-B120 (1.8 %), and B/IU, J/IU, Ia/IU (0.9 % each) serotypes. Single- nucleotide polymorphisms (SNPs) of omp1 gene were detected in E, K, and G serotypes. Some of these SNPs (C/T at position 272 and G/A at position 813 in E strain ; C/T at position 884 in D strain) might represent novel omp1 variants. |
| Databáze: | OpenAIRE |
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