Molecular epidemiology of methicillin-resistant Staphylococcus aureus in Majorcan hospitals: high prevalence of the epidemic clone EMRSA-15

Autor: José Luis Monereo Pérez, C. Gallegos, M. Cruz Perez, Amparo Oliver, E. Ruiz de Gopegui, Ana L. Ramirez, José E Costa Gil, Emma Padilla, Ana Mena, Angela Bosch Serra, Eva Alcoceba
Rok vydání: 2007
Předmět:
DNA
Bacterial
Microbiology (medical)
Staphylococcus aureus
medicine.medical_specialty
Micrococcaceae
Clone (cell biology)
Bacteremia
methicillin-resistant Staphylococcus aureus
medicine.disease_cause
Clones
Microbiology
Drug Resistance
Multiple
Bacterial
Epidemiology
Prevalence
Pulsed-field gel electrophoresis
Cluster Analysis
Humans
Medicine
Deoxyribonucleases
Type II Site-Specific
Cross Infection
Molecular Epidemiology
biology
Molecular epidemiology
business.industry
General Medicine
Staphylococcal Infections
biology.organism_classification
DNA Fingerprinting
Methicillin-resistant Staphylococcus aureus
Virology
Hospitals
Majorca
Anti-Bacterial Agents
Electrophoresis
Gel
Pulsed-Field
virulence
Multiple drug resistance
Blood
Infectious Diseases
pulsed-field gel electrophoresis
Spain
epidemiology
Methicillin Resistance
business
Zdroj: Clinical Microbiology and Infection. 13(6):599-605
ISSN: 1198-743X
DOI: 10.1111/j.1469-0691.2007.01703.x
Popis: Clinical isolates ( n = 389) of methicillin-resistant Staphylococcus aureus (MRSA) recovered from 371 patients between January 2003 and June 2004 at the three major public hospitals on the island of Majorca, Spain were studied. The clonal relatedness of MRSA isolates was determined by pulsed-field gel electrophoresis (PFGE) after digestion with Sma I. During the study period, MRSA was found in 31 % of patients with S. aureus -positive cultures. PFGE analysis identified three predominant clones, affecting 94% of the patients. The three clones had been detected since 1999 in one hospital, and were designated as clones A, B and C. Whereas clones A and B (multidrug-resistant) were related to the two most prevalent clones in Spain at this time, clone C was identical to EMRSA-15, currently one of the most common MRSA clones in UK hospitals and also detected in other countries, but rarely in Spanish hospitals. This imported epidemic clone was detected in c. 10% of patients admitted to one of the three hospitals in 2002, but its prevalence has increased significantly (32% of the patients investigated in the three hospitals in the present study), and this clone also accounted for 44% of the isolates from non-hospitalised patients. Even though EMRSA-15 showed the least multidrug resistance of the three major clones, it was apparently more virulent, since it was associated significantly (p 0.001) with bacteraemia, and positive blood cultures were documented for 21 % of the patients infected by this clone, compared with only 10% and 7% of patients infected with clones A and B, respectively.
Databáze: OpenAIRE
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