Red-shifted click beetle luciferase mutant expands the multicolor bioluminescent palette for deep tissue imaging
| Autor: | Laura Mezzanotte, Clemens W G M Löwik, Lance P. Encell, Mary P. Hall, Yanto Ridwan, Natasa Gaspar, Thomas A. Kirkland, Giorgia Zambito, Ce Shi, Monika G. Wood, Fabio Stellari |
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| Přispěvatelé: | Molecular Genetics, Radiology & Nuclear Medicine |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Luciferases Multidisciplinary Click beetle biology Chemistry Mutant Optical Imaging Biophysics 02 engineering and technology 021001 nanoscience & nanotechnology biology.organism_classification Luciferin Article Biological Services 03 medical and health sciences 030104 developmental biology In vivo Bioluminescence Bioluminescence imaging Luciferase lcsh:Q 0210 nano-technology lcsh:Science |
| Zdroj: | iScience, Vol 24, Iss 1, Pp 101986-(2021) iScience, 24(1):101986. Elsevier Inc. iScience |
| ISSN: | 2589-0042 |
| Popis: | Summary For in vivo multicolor bioluminescence applications, red and near-infrared signals are desirable over shorter wavelength signals because they are not as susceptible to light attenuation by blood and tissue. Herein, we describe the development of a new click beetle luciferase mutant, CBG2, with a red-shifted color emission. When paired with NH2-NpLH2 luciferin, CBG2 (λ = 660 nm) and CBR2 (λ = 730 nm) luciferases can be used for simultaneous dual-color bioluminescence imaging in deep tissue. Using a spectral unmixing algorithm tool it is possible to distinguish each spectral contribution. Ultimately, this enzyme pair can expand the near-infrared bioluminescent toolbox to enable rapid visualization of multiple biological processes in deep tissue using a single substrate. Graphical Abstract Highlights • CBG2 is a new click beetle mutant derived from CBG99 luciferase • It has a far red-shifted bioluminescent emission with NH2-NpLH2 luciferin (λ = 660 nm) • CBG2 with CBR2 luciferase and NH2-NpLH2 allows fast acquisition of data in deep tissue Optical Imaging; Biological Services; Biophysics |
| Databáze: | OpenAIRE |
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