Molecular Mapping of a Recessive Powdery Mildew Resistance Gene in Wheat Cultivar Tian Xuan 45 Using Bulked Segregant Analysis with Polymorphic Single Nucleotide Polymorphism Relative Ratio Distribution
| Autor: | Dongfang Ma, Qiang Li, Bei Su, Lei Wu, Baotong Wang, Kaixiang Chao, Wenwen Su |
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| Rok vydání: | 2019 |
| Předmět: |
Genetic Markers
0106 biological sciences 0301 basic medicine Genes Recessive Single-nucleotide polymorphism Plant Science Biology Genes Plant Polymorphism Single Nucleotide 01 natural sciences Genetic analysis Chromosomes Plant Manhattan plot 03 medical and health sciences Podospora Cultivar Gene Triticum Disease Resistance Plant Diseases Genetics Bulked segregant analysis Chromosome Mapping food and beverages 030104 developmental biology Genetic marker Agronomy and Crop Science Powdery mildew 010606 plant biology & botany |
| Zdroj: | Phytopathology®. 109:828-838 |
| ISSN: | 1943-7684 0031-949X |
| DOI: | 10.1094/phyto-03-18-0092-r |
| Popis: | Powdery mildew is a destructive foliar disease of wheat worldwide. Wheat cultivar Tian Xuan 45 exhibits resistance to the highly virulent isolate HY5. Genetic analysis of the F2 and F2:3 populations of a cultivar Ming Xian 169/Tian Xuan 45 cross revealed that the resistance to HY5 was controlled by a single recessive gene, temporarily designated as PmTx45. A Manhattan plot with the relative frequency distribution of single nucleotide polymorphisms (SNPs) was used to rapidly narrow down the possible chromosomal regions of the associated genes. This microarray-based bulked segregant analysis (BSA) largely improved traditional analytical methods. PmTx45 was located in chromosomal bin 4BL5-0.86-1.00 and was flanked by SNP marker AX-110673642 and intron length polymorphism (ILP) marker ILP-4B01G269900 with genetic distances of 3.0 and 2.6 cM, respectively. Molecular detection in a panel of wheat cultivars using the markers linked to PmTx45 showed that the presence of PmTx45 in commercial wheat cultivars was rare. Resistance spectrum and chromosomal position analyses indicated that PmTx45 may be a novel recessive gene with moderate powdery mildew resistance. This new microarray-based BSA method is feasible and effective and has the potential application for mapping genes in wheat in marker-assisted breeding. |
| Databáze: | OpenAIRE |
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