Osteoblast activity on anodized titania nanotubes: Effect of simulated body fluid soaking time
| Autor: | Emir Baki Denkbaş, Melike Erol Demirbilek, Nazli Calişkan, Cem Bayram, Murat Demirbilek |
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| Přispěvatelé: | Sabire Yazıcı Fen Edebiyat Fakültesi |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Materials science
Cell Survival Surface Properties Simulated body fluid Biomedical Engineering Molecular Conformation Titania Nanotube Pharmaceutical Science Medicine (miscellaneous) chemistry.chemical_element Bioengineering engineering.material Osseointegration Apatite Hydroxyapatite Simulated Body Fluid Coating Biomimetic Materials Osteogenesis Materials Testing Cell Adhesion Humans General Materials Science Particle Size Electrodes Cells Cultured Titanium Nanotubes Osteoblasts Anodizing Osteoblast Metallurgy Body Fluids Chemical engineering chemistry visual_art engineering visual_art.visual_art_medium Surface modification Implant Anodization |
| Popis: | Bayram, Cem (Aksaray, Yazar) Erol Demirbilek, Melike (Aksaray, Yazar) Early phase osseointegration is crucial for orthopedic implants. For the improvement of osseointegrative properties of orthopedic implants several surface modification methods such as acid etching, hydroxyapatite (HA) coating and sandblasting can be applied. In this article titanium implants were anodized to possess nanotubular titania structures on the surface. Titania nanotube structures with a 45-50 nm of average inner diameter were obtained and to enhance bioactivity, samples were soaked in 10X simulated body fluid (SBF) for apatite deposition on surface for different time periods (1, 2, 3, 5, 8 hours). Apatitic calcium phosphate deposited surfaces were analyzed with infrared spectrometry and wettability studies. Effect of soaking time on osteoblast cell was investigated by cell viability, alkaline phosphatase activity tests and morphological evaluations. As a result, 3 hours of soaking time was found as the optimum time period (p < 0.005). This in vitro study indicated that soaking in 10X SBF can be a rapid and economical technique to enhance osseointegration of anodized titanium implants however excess and/or uncontrolled HA coating of titania layer limits the bioactive potential of the implant. |
| Databáze: | OpenAIRE |
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