Muscleblind-like 1 antisense RNA 1 inhibits cell proliferation, invasion, and migration of prostate cancer by sponging miR-181a-5p and regulating PTEN/PI3K/AKT/mTOR signaling
| Autor: | Chuang Chen, Sai Su, Ye Yuan, Song-Tao Xu, Xu Xu, Xin-Yu Shen, Zhang Chen, Yuhua Huang, Xue-Feng He, Xiang Ding |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Bioengineering mbnl1-as1 migration Applied Microbiology and Biotechnology Phosphatidylinositol 3-Kinases 03 medical and health sciences 0302 clinical medicine Cell Movement Cell Line Tumor microRNA Humans PTEN Tensin RNA Antisense Protein kinase B PI3K/AKT/mTOR pathway Cell Proliferation biology Cell growth Chemistry TOR Serine-Threonine Kinases PTEN Phosphohydrolase Prostatic Neoplasms General Medicine Transfection prostate cancer invasion Antisense RNA pten MicroRNAs 030104 developmental biology 030220 oncology & carcinogenesis Cancer research biology.protein Proto-Oncogene Proteins c-akt TP248.13-248.65 Signal Transduction Research Article Research Paper Biotechnology |
| Zdroj: | Bioengineered, Vol 12, Iss 1, Pp 803-814 (2021) Bioengineered article-version (VoR) Version of Record |
| ISSN: | 2165-5987 2165-5979 |
| DOI: | 10.1080/21655979.2021.1890383 |
| Popis: | The present study aimed to investigate the role and underlying mechanisms of long non-coding RNA (lncRNA) muscleblind-like 1 antisense RNA 1 (MBNL1-AS1) in the progression of Prostate cancer (PCa). MBNL1-AS1 and microRNA (miR)-181a-5p expression in PCa tissues and several human PCa cell lines were analyzed, respectively, using StarBasev3.0 project and RT-qPCR assay. After MBNL1-AS1 overexpression, cell proliferation, invasion and migration were, respectively, evaluated using CCK-8, colony formation, transwell and wound healing assays. Dual luciferase assay were used for analysis of the interactions among MBNL1-AS1, miR-181a-5p, and phosphatase and tensin homolog (PTEN). Subsequently, the expression of PTEN and proteins in PI3K/AKT/mTOR signaling was examined using western blot analysis after transfection with miR-181a-5p mimic. The rescue assays were performed to investigate the effects of MBNL1-AS1 and miR-181a-5p on the functions of PCa cells and the expression of PTEN/PI3K/AKT/mTOR signaling by co-transfection with MBNL1-AS1 plasmid and miR-181a-5p mimic. Results indicated that MBNL1-AS1 was conspicuously downregulated while miR-181a-5p upregulating in PCa tissues and cell lines. MBNL1-AS1 overexpression decreased the abilities of cell proliferation, invasion, and migration. Further study revealed that MBNL1-AS1 acted as a sponge for miR-181a-5p and positively regulated PTEN by a sponge effect. Additionally, rescue assays proved that the effect of MBNL1-AS1-upregulation on the proliferation, invasion, and migration of PCa cells was dependent on miR-181a-5p. Furthermore, miR-181a-5p overexpression counteracted the expression of PTEN and proteins in PI3K/AKT/mTOR signaling exerted by MBNL1-AS1-upregulation in PCa cells. This study suggests that MBNL1-AS1 inhibits the progression of PCa via sponging miR-181a-5p and regulating PTEN/PI3K/AKT/mTOR pathway. GRAPHICAL ABSTRACT |
| Databáze: | OpenAIRE |
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