Virtual Crossmatch in Kidney Transplantation
| Autor: | D. Caputo, Elvira Poggi, Antonina Piazza, G. Ozzella, A. R. Manfreda, Domenico Adorno |
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| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Urology Kidney transplant Antibodies Donor Selection Hla molecules HLA Antigens medicine Humans Kidney transplantation Retrospective Studies Transplantation business.industry Donor selection Histocompatibility Testing Mean fluorescence intensity Middle Aged Flow Cytometry medicine.disease Serum samples Kidney Transplantation Surgery body regions Lazio region Italy Female business |
| Zdroj: | Transplantation proceedings 46 (2014): 2195–2198. doi:10.1016/j.transproceed.2014.07.053 info:cnr-pdr/source/autori:Piazza, A.; Ozzella, G.; Poggi, E.; Caputo, D.; Manfreda, A.; Adorno, D./titolo:Virtual Crossmatch in Kidney Transplantation/doi:10.1016%2Fj.transproceed.2014.07.053/rivista:Transplantation proceedings/anno:2014/pagina_da:2195/pagina_a:2198/intervallo_pagine:2195–2198/volume:46 |
| ISSN: | 0041-1345 |
| DOI: | 10.1016/j.transproceed.2014.07.053 |
| Popis: | BACKGROUND: The Luminex Single-Antigen Beads (LSA) assay allows an accurate detection and characterization of preexisting donor-specific antibodies (DSA) in kidney transplant candidates. But the ability of LSA to detect quite low levels of antibodies makes it hard to correctly predict crossmatch results in donor selection. In this study we retrospectively analyzed the accuracy of our virtual crossmatch (v-XM) protocol, which was used for selection of potential kidney transplant recipients, in predicting the results of actual crossmatch (a-XM) in cadaver-donor renal transplantation. We also investigated correlation between negative a-XM results and strength/specificity of preformed DSA. METHODS: The correlation between negative v-XMs and a-XMs performed in 2007-2012 at the Regional Transplant Center of the Lazio Region, Italy, was analyzed. In carrying out v-XM, the donor HLA molecules against which patients showed LSA-detected DSA with normalized mean fluorescence intensity (MFI)>=5,000 were considered to be "unacceptable DSA," and LSA-DSA showing MFI |
| Databáze: | OpenAIRE |
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