Functional Divergence of Poplar Histidine-Aspartate Kinase HK1 Paralogs in Response to Osmotic Stress
| Autor: | Mirai Tanigawa, Mélanie Larcher, Inès Djeghdir, Daniel Auguin, Franck Coste, François Héricourt, Christiane Depierreux, Florent Lafontaine, Gaëlle Glévarec, Sabine Carpin, Vincent Courdavault, Françoise Chefdor, Tatsuya Maeda |
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| Přispěvatelé: | ProdInra, Archive Ouverte, Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Institut National de la Recherche Agronomique (INRA)-Université d'Orléans (UO), Biomolécules et biotechnologies végétales (BBV EA 2106), Université de Tours (UT), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Tours, Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine 01 natural sciences Substrate Specificity lcsh:Chemistry histidine-aspartate kinase (HK) histidine-containing phosphotransfer protein (HPt) multistep phosphorelay (MSP) osmosensing Populus drought signaling duplication de gènes Gene Expression Regulation Plant Protein-fragment complementation assay Gene Duplication [SDV.BV] Life Sciences [q-bio]/Vegetal Biology Amino Acids lcsh:QH301-705.5 Phylogeny Spectroscopy chemistry.chemical_classification Genetics sodium chloride stress Vegetal Biology phosphorylation histidine kinase General Medicine Computer Science Applications Amino acid Biochemistry adaptation à la sécheresse Phosphorylation Protein Binding protéine de transfert aspartate kinase aspartyl kinase populus Biology gène paralogue stress osmotique Article Catalysis Inorganic Chemistry 03 medical and health sciences Osmotic Pressure Stress Physiological Two-Hybrid System Techniques [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Aspartate kinase Physical and Theoretical Chemistry Kinase activity Molecular Biology Histidine Aspartic Acid Sequence Homology Amino Acid Genetic Complementation Test Organic Chemistry Mutagenesis fungi Reproducibility of Results osmose tolérance à la sécheresse osmotic flow 030104 developmental biology chemistry lcsh:Biology (General) lcsh:QD1-999 Mutation Mutant Proteins Protein Multimerization Functional divergence Biologie végétale 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, 2016, 17 (12), ⟨10.3390/ijms17122061⟩ International Journal of Molecular Sciences, Vol 17, Iss 12, p 2061 (2016) International Journal of Molecular Sciences, MDPI, 2016, 17 (12), ⟨10.3390/ijms17122061⟩ International Journal of Molecular Sciences 12 (17), . (2016) International Journal of Molecular Sciences; Volume 17; Issue 12; Pages: 2061 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Previous works have shown the existence of protein partnerships belonging to a MultiStep Phosphorelay (MSP) in Populus putatively involved in osmosensing. This study is focused on the identification of a histidine-aspartate kinase, HK1b, paralog of HK1a. The characterization of HK1b showed its ability to homo- and hetero-dimerize and to interact with a few Histidine-containing Phosphotransfer (HPt) proteins, suggesting a preferential partnership in poplar MSP linked to drought perception. Furthermore, determinants for interaction specificity between HK1a/1b and HPts were studied by mutagenesis analysis, identifying amino acids involved in this specificity. The HK1b expression analysis in different poplar organs revealed its co-expression with three HPts, reinforcing the hypothesis of partnership participation in the MSP in planta. Moreover, HK1b was shown to act as an osmosensor with kinase activity in a functional complementation assay of an osmosensor deficient yeast strain. These results revealed that HK1b showed a different behaviour for canonical phosphorylation of histidine and aspartate residues. These phosphorylation modularities of canonical amino acids could explain the improved osmosensor performances observed in yeast. As conserved duplicates reflect the selective pressures imposed by the environmental requirements on the species, our results emphasize the importance of HK1 gene duplication in poplar adaptation to drought stress. |
| Databáze: | OpenAIRE |
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