Diagnostic value of human papillomavirus (HPV) 16 and HPV18 viral loads for the detection of high-grade cervical intraepithelial neoplasia (CIN2+) in a cohort of African women living with HIV

Autor: Helen Kelly, Sylviane Doutre, Olga Goumbri-Lompo, Marie-Noelle Didelot, Michel Segondy, Philippe Mayaud, Tanvier Omar, Jean Ngou
Přispěvatelé: Pathogénèse et contrôle des infections chroniques (PCCI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ), Hôpital Lapeyronie [Montpellier] (CHU), Université de Montpellier (UM), London School of Hygiene and Tropical Medicine (LSHTM), CHU Montpellier, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier )-Université de Montpellier (UM)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Oncology
viruses
Uterine Cervical Neoplasms
HIV Infections
Cervix Uteri
0302 clinical medicine
Viral Envelope Proteins
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Viral load
Prospective Studies
Cervical cancer
education.field_of_study
Human papillomavirus 16
medicine.diagnostic_test
Human papillomavirus 18
Coinfection
virus diseases
Middle Aged
female genital diseases and pregnancy complications
3. Good health
Infectious Diseases
medicine.anatomical_structure
030220 oncology & carcinogenesis
Cohort
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Female
Adult
HPV16
medicine.medical_specialty
Adolescent
Population
HPV18
[SDV.MHEP.GEO]Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics
Cervical intraepithelial neoplasia
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
03 medical and health sciences
Young Adult
Virology
Internal medicine
Biopsy
medicine
Humans
education
Cervix
Africa South of the Sahara
business.industry
Papillomavirus Infections
medicine.disease
Uterine Cervical Dysplasia
030104 developmental biology
High Grade Cervical Intraepithelial Neoplasia
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
business
Zdroj: Journal of Clinical Virology
Journal of Clinical Virology, Elsevier, 2018, 99-100, pp.79-83. ⟨10.1016/j.jcv.2018.01.006⟩
ISSN: 1386-6532
DOI: 10.1016/j.jcv.2018.01.006⟩
Popis: International audience; Background: African women living with HIV (WLHIV) are at high risk of cervical cancer but rarely adequately screened. Better strategies enabling identification of WLHIV with high-grade cervical intraepithelial lesions (CIN2+) are required. Objectives: To investigate the diagnostic value of HPV16 and HPV18 viral loads in a cohort of African WLHIV. Design: HPV16 and HPV18 viral loads were determined by quantitation of the E6 gene DNA by real-time PCR in cervical specimens collected at baseline and endline (16 months) from 245 African WLHIV positive for HPV16 or/and HPV18. Cervical biopsies were graded using the histopathological CIN classification. Results: Women with CIN2+ had higher viral load for HPV16 (p < 0.0001) or HPV18 (p = 0.03) than those without CIN2+. HPV16 viral load ≥3.59 log copies/1000 cells detected CIN2+ with sensitivity and specificity of 93.5% (95%CI: 81.7-98.3%) and 74.1% (95%CI: 66.3-80.6%), respectively, whereas HPV18 viral load ≥1.63 log copies/1000 cells detected CIN2+ with sensitivity and specificity of 59.1% (95%CI: 38.7-76.7%) and 66.9% (95%CI: 58.8-74.1%), respectively. A high baseline HPV16 viral load was significantly associated with persistence of, or progression to CIN2+ at endline; these findings were not observed for HPV18. Conclusions: HPV16 viral load is a powerful marker of CIN2+ in African WLHIV. HPV18 viral load is of lower diagnostic value in this population.
Databáze: OpenAIRE
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