Effect of mechanical loading and substrate elasticity on the osteogenic and adipogenic differentiation of mesenchymal stem cells
| Autor: | X. Frank Walboomers, John A. Jansen, Min Bao, Hatice Imran Gungordu, Sander C.G. Leeuwenburgh, Sjoerd van Helvert |
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| Rok vydání: | 2019 |
| Předmět: |
Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2]
Cellular differentiation 0206 medical engineering Biomedical Engineering Acrylic Resins Medicine (miscellaneous) 02 engineering and technology Rare cancers Radboud Institute for Molecular Life Sciences [Radboudumc 9] Biomaterials Extracellular matrix 03 medical and health sciences Osteogenesis Extracellular Animals Dimethylpolysiloxanes Elasticity (economics) 030304 developmental biology 0303 health sciences Adipogenesis Chemistry Mesenchymal stem cell Biomaterial Cell Differentiation Mesenchymal Stem Cells 020601 biomedical engineering Elasticity Rats Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] Biophysics Alkaline phosphatase Physical Organic Chemistry |
| Zdroj: | Journal of Tissue Engineering and Regenerative Medicine, 13, 12, pp. 2279-2290 Journal of Tissue Engineering and Regenerative Medicine, 13, 2279-2290 |
| ISSN: | 1932-6254 |
| DOI: | 10.1002/term.2956 |
| Popis: | Contains fulltext : 215424.pdf (Author’s version postprint ) (Open Access) Mesenchymal stem cells (MSCs) are highly sensitive to biomechanics of their extracellular environment. Generally, a higher elasticity of culture substrates can drive cells into the osteogenic lineage, whereas low substrate elasticity results in adipogenesis. Applied mechanical loading by cyclic strain is another major variable influencing cell fate. Yet, little is known about the simultaneous effect of both cues. Therefore, the present study investigated the relative importance of both cues on differentiation. MSCs were cultured in an osteogenic and also an adipogenic environment on soft polyacrylamide (PAAm; E = 23 +/- 0.3 kPa), stiff PAAm (111 +/- 2 kPa), and polydimethylsiloxane (PDMS; E = 1,5 +/- 0.07 MPa) either unstrained or with 8% cyclic strain at 1 Hz. Without strain, the relative expression of the early osteogenic marker alkaline phosphatase (ALP) was significantly higher (78%) on PDMS than on both PAAm. With 8% cyclic strain, ALP expression increased for all groups in comparison with unstrained controls. The highest increase was observed for the soft PAAm by 36%. Moreover, relative oil red O (ORO) expression-indicating adipogenesis-was the highest for unstrained soft PAAm. On the other hand, the percentage of ORO positive cells significantly decreased by 57% and 69% for soft and stiff PAAm when strained. In conclusion, biomaterial elasticity and mechanical loading can act simultaneously on cell differentiation. Substrate elasticity is an important factor, regulating the differentiation, but cyclic strain can drive MSCs towards the osteogenesis even on the softest substrate. As such, the osteogenic effect of mechanical loading can overrule the adipogenic effect of soft substrates, thereby acting as an inhibitor. |
| Databáze: | OpenAIRE |
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