Effects of enhancer mutations on the expression of human immunodeficiency virus 1-regulated luciferase and diphtheria toxin A chain genes in transfected cells
| Autor: | Gail S. Harrison, Inmaculada Castillo, Cynthia J. Long |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Gene Expression Regulation
Viral Regulation of gene expression Diphtheria toxin Mutation Transfection Biology Toxicology HIV Enhancer medicine.disease_cause Virology Molecular biology Peptide Fragments Enhancer Elements Genetic Gene expression HIV-1 medicine Humans Diphtheria Toxin Luciferase Luciferases Enhancer HeLa Cells Plasmids |
| Zdroj: | Toxicon. 31:85-90 |
| ISSN: | 0041-0101 |
| DOI: | 10.1016/0041-0101(93)90360-u |
| Popis: | This study explores human immunodeficiency virus 1 (HIV-1)-regulated diphtheria toxin A (DT-A) gene expression as a means of eradicating HIV-infected cells. Previously, we constructed luciferase and DT-A plasmids, containing cis-acting Tat and Rev responsive elements, which showed low basal expression and required both Tat and Rev for maximal expression. Cell lines which had stably integrated the DT-A constructs were resistant to HIV production. To reduce toxicity due to basal expression, this study investigates the effect of mutations in the HIV enhancer on expression of luciferase and DT-A plasmids. Some mutations were found to substantially reduce basal expression while still allowing for trans-activation. Such mutations, in combination with attenuated versions of DT-A, may make regulated toxin gene expression feasible as a therapy for AIDS. |
| Databáze: | OpenAIRE |
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