Changes in the lectin-binding pattern of PNA-agglutinin and UEA1during the DMH-induced carcinogenesis in the normal appearing colonic mucosa of the rat
| Autor: | F. Sandforth, T. Balzer, S. Gutschmidt, Ernst-Otto Riecken |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Peanut agglutinin
Pathology medicine.medical_specialty Colorectal cancer Clinical Biochemistry Rectum medicine.disease_cause Biochemistry Fluorescence Caecum Peanut Agglutinin chemistry.chemical_compound Agglutinin Lectins medicine Animals Intestinal Mucosa Fluorescein Dimethylhydrazines biology Mucin Mucins Rats Inbred Strains General Medicine Fluoresceins medicine.disease biology.organism_classification 1 2-Dimethylhydrazine Rats medicine.anatomical_structure chemistry Colonic Neoplasms biology.protein Female Plant Lectins Carcinogenesis Fluorescein-5-isothiocyanate Thiocyanates |
| Zdroj: | European Journal of Clinical Investigation. 18:196-201 |
| ISSN: | 0014-2972 |
| DOI: | 10.1111/j.1365-2362.1988.tb02413.x |
| Popis: | The purpose of this study was the investigation of changes in the lectin-binding pattern prior to tumour formation in an experimental model. Female Wistar rats were treated with 1,2-dimethylhydrazine (DMH). After 4, 8, 16, 24, 32 and 40 weeks of treatment the lectin-binding-pattern of the colonic mucosa appearing morphologically normal was examined at the caecum, proximal colon, distal colon and rectum, using FITC-conjugated Peanut-agglutinin (PNA) and Ulex europaeus-agglutinin1 (UEA1). In contradistinction to what has been reported earlier by other authors, PNA did not indicate constant cancer-associated mucin changes. In addition, there was no difference in the UEA1-binding between the control animals and the DMH-treated rats. Thus, in the rat there is no specific PNA- and UEA1-binding pattern during tumour induction in the gut. |
| Databáze: | OpenAIRE |
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