Accumulation of amino-polyvinyl alcohol-coated superparamagnetic iron oxide nanoparticles in bone marrow: implications for local stromal cells

Autor: Heinrich Hofmann, Frank Schulze, Lindsey A. Crowe, Jean-Paul Vallée, Azza Gramoun, Anke Dienelt, Andrea Ode, Georg N. Duda, Tugba Akcan
Předmět:
Nanoparticles/chemistry/metabolism
Pathology
medicine.medical_specialty
Contrast enhancement
Materials science
Stromal cell
bone marrow
Superparamagnetic iron oxide nanoparticles
Contrast Media/chemistry/metabolism
Biomedical Engineering
Medicine (miscellaneous)
Contrast Media
Bioengineering
Development
ddc:616.0757
Polyvinyl alcohol
Ferric Compounds
chemistry.chemical_compound
Ferric Compounds/chemistry
In vivo
medicine
Animals
General Materials Science
Bone Marrow/metabolism
integumentary system
superparamagnetic iron oxide nanoparticles
Histology
Mesenchymal Stem Cells
In vitro
Cell biology
Rats
medicine.anatomical_structure
polyvinyl alcohol
chemistry
Mesenchymal Stromal Cells/metabolism
Nanoparticles
contrast enhancement
Polyvinyl Alcohol/chemistry
Bone marrow
bone marrow stromal cells
MRI
Zdroj: Nanomedicine, Vol. 10, No 14 (2015) pp. 2139-51
ISSN: 1549-9634
Popis: Aims: First, it will be investigated if amino-polyvinyl alcohol-coated superparamagnetic iron oxide nanoparticles (A-PVA-SPIONs) are suitable for MRI contrast enhancement in bone marrow. Second, the impact of A-PVA-SPION exposure in vivo on the viability and key functions of local bone marrow stromal cells (BMSCs) will be investigated. Material & methods: Animals were systemically injected with A-PVA-SPIONs, followed by a 7-day survival time. Accumulation of A-PVA-SPIONs was confirmed by MRI, histology and inductively coupled plasma optical emission spectrometry. BMSCs were isolated from bone marrow for in vitro assessment of their viability and regenerative key functions. Results: In this study, A-PVA-SPIONs were found to accumulate in bone marrow and increase the BMSCs’ metabolic activity and migration rate. Conclusion: A-PVA-SPIONs appear suitable for contrast enhancement in bone marrow while our data suggest an influence on the BMSCs biology that necessitates future research.
Databáze: OpenAIRE
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