Mediterranean-like mix of fatty acids induces cellular protection on lipid-overloaded hepatocytes from western diet fed mice
| Autor: | Luis Enrique Gómez-Quiroz, Lyssia Castellanos-Tapia, María Elizabeth Tejero-Barrera, Alejandro Escobedo-Calvario, Arturo Simoni-Nieves, Soraya Salas-Silva |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Specialties of internal medicine Diet Mediterranean 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Antigens Neoplasm Non-alcoholic Fatty Liver Disease Mediterranean diet NAFLD medicine TBARS Animals MUFA Food science Western diet Cell damage Unsaturated fatty acid Cells Cultured Triglycerides chemistry.chemical_classification Mice Inbred BALB C Hepatology Cholesterol business.industry Fatty liver Fatty acid General Medicine medicine.disease Lipid Metabolism Phospholipases A1 Disease Models Animal Oxidative Stress RC581-951 chemistry Liver Diet Western 030220 oncology & carcinogenesis Fatty Acids Unsaturated Hepatocytes 030211 gastroenterology & hepatology Steatosis business Reactive Oxygen Species PUFA Polyunsaturated fatty acid |
| Zdroj: | Annals of Hepatology, Vol 19, Iss 5, Pp 489-496 (2020) |
| ISSN: | 1665-2681 |
| Popis: | Introduction and objective. Non-alcoholic fatty liver disease remains as one of the main liver disorders worldwide. It is widely accepted that is the kind of lipid, rather than the amount deposited in the cells that determines cell damage. Cholesterol and saturated free fatty acids are deleterious lipids when accumulated but, in contrast, there are some valuable lipids that could counteract those with harmful properties. Much of this knowledge arises from studies using a single fatty acid, but the effects of a combination of fatty acids, as obtained by diet has been poorly addressed. In the present work, we were focused to figure out the cellular effect of two different mixes of fatty acids, one with high proportion of saturated fatty acids, and another one with high proportion of unsaturated fatty acids (Mediterranean-like) in a cellular model of steatosis. Material and methods. Primary mouse hepatocytes from animals fed with a western diet (high fat and carbohydrates diet), were treated with both mixes of fatty acids for 24 h. Results. Our data clearly show that only the high unsaturated fatty acid mix induced a decrease in triglycerides (47.5%) and cholesterol (59%) content in steatotic hepatocytes mediating cellular protection associated to the decrement of ROS and oxidative damage. The mixture of high saturated fatty acids exhibited no effects, preserving high levels of cholesterol and triglycerides and oxidative damage. In conclusion, our results show that Mediterranean-like mix of fatty acids exerts cellular protection in steatosis by decreasing triglycerides, cholesterol, ROS content and oxidative damage. |
| Databáze: | OpenAIRE |
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