Seroepidemiological survey of human exposure to Dirofilaria spp. in Romania and Moldova
Autor: | Giuseppe Cringoli, Marco Genchi, Liviu Miron, Fernando Simón, Rodrigo Morchón, Lavinia Ciuca, Dumitru Acatrinei, Laura Rinaldi, Laura D. Kramer |
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Přispěvatelé: | Ciuca, Lavinia, Simòn, Fernando, Rinaldi, Laura, Kramer, Laura, Genchi, Marco, Cringoli, Giuseppe, Acatrinei, Dumitru, Miron, Liviu, Morchon, Rodrigo |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Adult Male Veterinary medicine Adolescent Veterinary (miscellaneous) Dirofilaria immitis 030231 tropical medicine Antibodies Helminth Enzyme-Linked Immunosorbent Assay Repens Serology 03 medical and health sciences Young Adult 0302 clinical medicine Dogs Antigen Human dirofilariosis ELISA Dirofilaria immitis Dirofilaria repens Romania Moldova Seroepidemiologic Studies Dirofilariasis Surveys and Questionnaires medicine Prevalence Animals Humans Dog Diseases Child Dirofilaria Aged Aged 80 and over biology Romania 030108 mycology & parasitology Middle Aged Moldova biology.organism_classification medicine.disease Dirofilaria repens Infectious Diseases Insect Science Antigens Helminth Parasitology Female Wolbachia |
Zdroj: | Acta tropica. 187 |
ISSN: | 1873-6254 |
Popis: | The present study aimed to evaluate the extent of Dirofilaria immitis and D. repens exposure in humans from eastern and southern areas of Romania and central Moldova by serological methods. The serological screening was performed on a total of 450 serum samples (187 from Romania and 263 from Moldova). The sera were collected using a convenience sampling with the help of physicians from the hospitals of the study areas. All samples were analysed by a non-commercial ELISA test for the detection of IgG antibodies against adult somatic antigens of D. immitis and D. repens. The results showed a total of 49 (10.9%; 95% CI = 8.3-14.1%) individuals from Romania and Moldova with a positive response to IgG antibodies against both adult somatic antigens of D. immitis and D. repens. Specifically, 48 (10.7%; 95% CI = 8.0-14.0%) patients were positive for IgG-antibodies against adult somatic antigens of D. immitis, one (0.2%; 95% CI = 0.4-1.2%) against D. repens antigens, and four (0.9%; 95% CI = 0.4-3.3%). were positive for antigens of both parasites. At country level, out of 187 samples from Romania, 13 (6.9%; 95% CI = 4.1-11.5%) were positive for anti-D. immitis IgG with high exposure in the southern part of the country (Bucharest). Of the 263 people from Moldova, 36 (13.7%; 95% CI = 10.0-18.4%) were positive for D. immitis antigens from which three (1.1%, 95% CI = 0.4-3.3%) were positive for the antibodies against antigens of both parasites. Only one sample was found positive for anti-D. repens IgG. Positive IgG-ELISA results were confirmed by Western blot analysis. In addition, for further confirmation, a complementary ELISA was performed for anti-WSP IgG antibodies against Wolbachia endosymbionts. Our findings showed a noticeable exposure of humans from Romania and Moldova to Dirofilaria parasites. Serology can be useful for indicating exposure to Dirofilaria spp. in a healthy population in order to obtain useful data on the epidemiological scenario of human dirofilariosis in Eastern Europe. |
Databáze: | OpenAIRE |
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