Encapsulation of grape seed extract in polylactide microcapsules for sustained bioactivity and time-dependent release in dental material applications
| Autor: | Ariene A. Leme-Kraus, Scott R. White, Berdan Aydin, Mostafa Yourdkhani, Ana K. Bedran-Russo |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Materials science
food.ingredient Scanning electron microscope Polyesters Capsules 02 engineering and technology Article 03 medical and health sciences Dental Materials 0302 clinical medicine food Dentin medicine General Materials Science Composite material General Dentistry Elastic modulus Chromatography Grape Seed Extract 030206 dentistry 021001 nanoscience & nanotechnology Biodegradable polymer Molar Solvent Polyester medicine.anatomical_structure Proanthocyanidin Mechanics of Materials Grape seed extract 0210 nano-technology |
| Popis: | Objective To sustain the bioactivity of proanthocyanidins-rich plant-derived extracts via encapsulation within biodegradable polymer microcapsules. Methods Polylactide microcapsules containing grape seed extract (GSE) were manufactured using a combination of double emulsion and solvent evaporation techniques. Microcapsule morphology, size distribution, and cross-section were examined via scanning electron microscopy. UV–vis measurements were carried out to evaluate the core loading and encapsulation efficiency of microcapsules. The bioactivity of extracts was evaluated after extraction from capsules via solvent partitioning one week or one year post-encapsulation process. Fifteen human molars were cut into 7 mm × 1.7 mm × 0.5 mm thick mid-coronal dentin beams, demineralized, and treated with either encapsulated GSE, pristine GSE, or left untreated. The elastic modulus of dentin specimens was measured based on three-point bending experiments as an indirect assessment of the bioactivity of grape seed extracts. The effects of the encapsulation process and storage time on the bioactivity of extracts were analyzed. Results Polynuclear microcapsules with average diameter of 1.38 μm and core loading of up to 38 wt% were successfully manufactured. There were no statistically significant differences in the mean fold increase of elastic modulus values among the samples treated with encapsulated or pristine GSE (p = 0.333), or the storage time (one week versus one year storage at room temperature, p = 0.967). Significance Polynuclear microcapsules containing proanthocyanidins-rich plant-derived extracts were prepared. The bioactivity of extracts was preserved after microencapsulation. |
| Databáze: | OpenAIRE |
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