The microdissected gene expression landscape of nasopharyngeal cancer reveals vulnerabilities in FGF and noncanonical NF-κB signaling
| Autor: | Joshua K. Tay, Chunfang Zhu, June Ho Shin, Shirley X. Zhu, Sushama Varma, Joseph W. Foley, Sujay Vennam, Yim Ling Yip, Chuan Keng Goh, De Yun Wang, Kwok Seng Loh, Sai Wah Tsao, Quynh-Thu Le, John B. Sunwoo, Robert B. West |
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| Rok vydání: | 2022 |
| Předmět: |
Epstein-Barr Virus Infections
Herpesvirus 4 Human Nasopharyngeal Carcinoma Multidisciplinary Intracellular Signaling Peptides and Proteins NF-kappa B Gene Expression Membrane Proteins Nasopharyngeal Neoplasms Fibroblast Growth Factors stomatognathic diseases Cell Line Tumor Tumor Microenvironment otorhinolaryngologic diseases Humans Signal Transduction |
| Zdroj: | Science Advances. 8 |
| ISSN: | 2375-2548 |
| Popis: | Nasopharyngeal cancer (NPC) is an Epstein-Barr virus (EBV)–positive epithelial malignancy with an extensive inflammatory infiltrate. Traditional RNA-sequencing techniques uncovered only microenvironment signatures, while the gene expression of the tumor epithelial compartment has remained a mystery. Here, we use Smart-3SEQ to prepare transcriptome-wide gene expression profiles from microdissected NPC tumors, dysplasia, and normal controls. We describe changes in biological pathways across the normal to tumor spectrum and show that fibroblast growth factor (FGF) ligands are overexpressed in NPC tumors, while negative regulators of FGF signaling, including SPRY1, SPRY2, and LGALS3, are down-regulated early in carcinogenesis. Within the NF-κB signaling pathway, the critical noncanonical transcription factors, RELB and NFKB2, are enriched in the majority of NPC tumors. We confirm the responsiveness of EBV-positive NPC cell lines to targeted inhibition of these pathways, reflecting the heterogeneity in NPC patient tumors. Our data comprehensively describe the gene expression landscape of NPC and unravel the mysteries of receptor tyrosine kinase and NF-κB pathways in NPC. |
| Databáze: | OpenAIRE |
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