Increase in alveolar antioxidant levels in hyperoxic and anoxic ventilated rabbit lungs during ischemia
| Autor: | Friedrich Grimminger, Christoph Schafer, Alexander Löckinger, Gerd Hermle, T Luboeinski, Werner Seeger, Andreas Günther, Reinhold Schmidt, Hartwig Schütte, Ardeschir Ghofrani |
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| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Plasmalogens Ischemia Lung injury Hyperoxia medicine.disease_cause Biochemistry Antioxidants chemistry.chemical_compound Physiology (medical) Internal medicine medicine Animals Hypoxia Lung medicine.diagnostic_test L-Lactate Dehydrogenase Chemistry Vitamin E Pulmonary Surfactants Glutathione Vitamins medicine.disease Ascorbic acid Uric Acid Endocrinology Bronchoalveolar lavage Anesthesia Breathing Rabbits Pulmonary Ventilation Bronchoalveolar Lavage Fluid Oxidation-Reduction Oxidative stress |
| Zdroj: | Free radical biologymedicine. 36(1) |
| ISSN: | 0891-5849 |
| Popis: | Increases in free radicals are believed to play a central role in the development of pulmonary ischemia/reperfusion (I-R) injury, leading to microvascular leakage and deterioration of pulmonary surfactant. Continued ventilation during ischemia offers significant protection against I-R injury, but the impact of alveolar oxygen supply both on lung injury and on radical generation is still unclear. We investigated the influence of hyperoxic (95% O2) and anoxic (0% O2) ventilation during ischemia on alveolar antioxidant status and surfactant properties in isolated rabbit lungs. Normoxic and hyperoxic ventilated, buffer-perfused lungs (n = 5 or 6) and native lungs (n = 6) served as controls. As compared with controls, biophysical and biochemical surfactant properties were not altered in anoxic as well as hyperoxic ventilated ischemic (2, 3, and 4 h) lungs. Assessment of several antioxidants (reduced glutathione (GSH), alpha-tocopherol (vitamin E), retinol (vitamin A), ascorbic acid (vitamin C), uric acid, and plasmalogens (1-O-alkenyl-2-acyl-phospholipids)) in bronchoalveolar lavage fluid (BALF) revealed a significant increase in antioxidant compounds under anoxic and hyperoxic ventilation, with maximum levels occuring after 3 h of ischemia. For example, GSH increased to 5.1 +/- 0.8 microM (mean +/- SE, p |
| Databáze: | OpenAIRE |
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